. Biological structure and function; proceedings. Biochemistry; Cytology. 62 F. EDMUND HUNTER, JR. iodoacetamide produce swelling, but it resembles that seen with phosphate more than that due to ascorbate. An interesting observation from these experiments is that very low ^-hydroxymercuribenzoate concentrations produce swelling after a lag period, while 60-100 /xM levels have a three- CONTROL. p-OH-MERCURIBENZOATE 10 20 30 40 MINUTES 50 60 Fig. 13. Absorbancy changes due to different concentrations of /)-hydroxy- mercuribenzoate. High concentrations show a three-phase curve
. Biological structure and function; proceedings. Biochemistry; Cytology. 62 F. EDMUND HUNTER, JR. iodoacetamide produce swelling, but it resembles that seen with phosphate more than that due to ascorbate. An interesting observation from these experiments is that very low ^-hydroxymercuribenzoate concentrations produce swelling after a lag period, while 60-100 /xM levels have a three- CONTROL. p-OH-MERCURIBENZOATE 10 20 30 40 MINUTES 50 60 Fig. 13. Absorbancy changes due to different concentrations of /)-hydroxy- mercuribenzoate. High concentrations show a three-phase curve. ^ â¢_-_ . CONTROLv, ^\'«> ""V^ - \\^ \^ ASCORBATE ^ â¢--.:.--rX-.,..â, 0 0. ImM /\\ \ *"""â -, CVJ in < p-OH-MERCURlBENZOATE\\ ^ "-.^ + ASCORBATE AT oO^\<^\ 0,2 - + ASCORBATE AT 20'-Av \ ⦠* â*~ â ~( 1 1 1 1 1 20 30 40 MINUTES 50 60 Fig. 14. Ascorbate added with or after p-hydroxymercuribenzoate produces fairly typical lysis. phase effectâfirst a rapid fall in absorbancy, then a definite plateau for about 10 min., then a further fall (Fig. 13). This may indicate two sites of action or an immediate and a delayed effect from a single site of action. Pyruvate seems to inhibit the initial phase with little effect on the second Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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