. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 1. L. callmectes is constricted as it penetrates the outer egg layers (arrow) of this unfertilized egg. Fungal hyphae invade the internal milieu and destroy the otherwise globular ooplasm. Toluidine blue stain, FIGURE 2. Unfertilized eggs were engulfed by fungal hyphae after three days incubation with L-Pm propagules while fertilized eggs suspended in the same flask were less susceptible. Even after five days, half the fertilized eggs remained uninfected. FIGURES 3 and 4. The fungal hyphae on unfertilized eggs
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 1. L. callmectes is constricted as it penetrates the outer egg layers (arrow) of this unfertilized egg. Fungal hyphae invade the internal milieu and destroy the otherwise globular ooplasm. Toluidine blue stain, FIGURE 2. Unfertilized eggs were engulfed by fungal hyphae after three days incubation with L-Pm propagules while fertilized eggs suspended in the same flask were less susceptible. Even after five days, half the fertilized eggs remained uninfected. FIGURES 3 and 4. The fungal hyphae on unfertilized eggs protruded directly outward from the egg surface whereas hyphae on fertilized eggs appeared to form a mat around the egg surface and the embryos may have been slowly asphyxiated rather than parasitized. infestation rather than infection. Instead of penetrating each embryo individually (where hyphae could be seen protruding from the embryo surface as in Figure 3), a hyphal mat grew around the cluster and appeared to wrap the embryos (Fig. 4). First appearance of the fungus varied from 1-3 days for unfertilized eggs. Inoculated samples usually required 3 days for first appearance unless nutrient was added, which reduced the time to infection for both eggs and embryos. Characteristics of the fungus, L-Pm The fungus L-Pm was determined to be Lagenidiaceous by virtue of its exten- sively branched, sparingly septate hyphae of 6-10 ^trn cross-sectional diameter. Its. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
