. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ISS REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS so- li 6°- c s £ 40-1 0. 1 I 270 310 350 390 430 470 510 550 590 630 Cornea + Lens — — Lens nm •- Cornea Visual pigment (OD) 1 0-4 H I Cornea Lens 270 290 310 330 350 370 390 410 430 450 4 4 M 4M a b c de f a c Figure 1. (A) Transmission spectra (left scale) through a /ens (5 mm in diameter, animal age is 5 months) and a cornea of a cuttlefish, each measured intact, and the combined transmission through cornea and lens: and normalized optical density (right scale) of the c
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ISS REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS so- li 6°- c s £ 40-1 0. 1 I 270 310 350 390 430 470 510 550 590 630 Cornea + Lens — — Lens nm •- Cornea Visual pigment (OD) 1 0-4 H I Cornea Lens 270 290 310 330 350 370 390 410 430 450 4 4 M 4M a b c de f a c Figure 1. (A) Transmission spectra (left scale) through a /ens (5 mm in diameter, animal age is 5 months) and a cornea of a cuttlefish, each measured intact, and the combined transmission through cornea and lens: and normalized optical density (right scale) of the cuttlefish visual pigment having a hmax at 490 nm (light-scatter by screening pigments anil possibly by visual products interfered with the signal at short wave- lengths and hence the spectra is plotted only at the 370-640 nm range). Transmission spectra show that the lens acts as a UV-A blocking filter with 50% transmission at about 365 nm. while the cornea's 50% cut- off is at about 295 nm. (B) Optical density of a cornea and a 40 urn thick section of a lens. Low levels of .scattering and higher absorption are revealed at wavelengths shorter than 390 nm. Arrows indicate \niax of compounds identified in the lens and cornea (a. 3-hydroxykynurenine 267 and 36N nm; h. unknown #l-295nm: c, unknown #2-324 and 3fi(> nm; d. palythinol-332 nm; e. shinorine-334 nm; f. palythene- 360 nm). Values demonstrate that the combined effect of these com- pounds is responsible for attenuating UV radiation. ensure the quality of the part of the sample examined. Fresh corneas and lenses were rapidly frozen, cut frozen, and thawed only on the measuring slide. Sivak (7| and Thorpe i't al. (2) found that this method does not distort the transmittance of light through the tissue. Additionally, a fresh lens. 5 mm in diameter, was examined within a specially prepared water well, using lOx Ultrafluar lenses as objective and condenser. Each sample was measured at 5 different locations. Each recor
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology