. Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 . Fig. 6. Identification of glutamic acid obtained from the wash solution of cells immersed in C"-glutamic acid. ^ is a photograph of the glutamic acid region of a paper chromatogram sprayed with ninhydrin. B shows the identical "fingerprint" obtained with a radioautograph of the same region of this chromatogram. The time required for the competitive exogenous supplements to replace endogenous synthesis has bee
. Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954 . Fig. 6. Identification of glutamic acid obtained from the wash solution of cells immersed in C"-glutamic acid. ^ is a photograph of the glutamic acid region of a paper chromatogram sprayed with ninhydrin. B shows the identical "fingerprint" obtained with a radioautograph of the same region of this chromatogram. The time required for the competitive exogenous supplements to replace endogenous synthesis has been studied using S^--cystine to compete with S^*04^. The rate at which the replacement of sulfate occurs is rapid, as shown by the sharp break in the radiosulfate uptake curve of figure 7. In this figure the ex- ponentially growing cells take radiosulfate at a rate proportional to growth. At the time of the addition of the nonradioactive cystine, the radiosulfate uptake ceases, but growth continues at the same optimal rate. The unlabeled cystine sulfur now supplies all sulfur requirements. That the carbon of the amino acid can also penetrate is shown by supplying nonlabeled cystine to medium containing C^-glucose as the only other carbon source. Under these conditions little of the C'' appears in the cystine of the proteins. This effective reduction of the biosynthesis of both the sulfur and the carbon of cystine when the exogenous amino acid is present suggests that the carbon and sulfur are
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