. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 496 D. RITTSCHOF ET FIGURE 1. Pipette bioassays for chemotactic response of juvenile snails (1-2 mm shell height) to barnacle stimulus. Snails in top pipette migrate upcurrent in sea water bearing barnacle scent, while those in lower pipette remain stationary in a current of filtered, scent-free sea water. Load zone indicated by A and bracket. Positive response criterion ml mark (B). snails were tested in two or more replicate runs for all test solutions. The standard assay procedure was improved by reducing the bac
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 496 D. RITTSCHOF ET FIGURE 1. Pipette bioassays for chemotactic response of juvenile snails (1-2 mm shell height) to barnacle stimulus. Snails in top pipette migrate upcurrent in sea water bearing barnacle scent, while those in lower pipette remain stationary in a current of filtered, scent-free sea water. Load zone indicated by A and bracket. Positive response criterion ml mark (B). snails were tested in two or more replicate runs for all test solutions. The standard assay procedure was improved by reducing the background stimulus activity of filtered sea water by allowing it to stand several days. Response of snails to back- ground cues was reduced four-fold from 20% to about 5% (G == P < ). This significant reduction in "noise" greatly improved the sensitivity of the assay. Experiments and observations Three series of experiments were performed. The first defined bioassay condi- tions. The effect of current velocities from zero to 360 cm/min was tested on the response of newly hatched snails to sea water with low endogenous attractant activity. Seven current velocities were examined with 100 snails each. The second series screened potential sources of attractant of Urosalpinx cinerea. It began with a mixed prey source and then tested individual species from that source. Finally other un- tested species were screened for attractant production. The mixed prey tests included: oysters (Crassostrea virginica), mussels (Mytilus edulis), barnacles (Balanus ebur- neus and Semibalanus balanoides), hydrozoans (Eudendrium sp.), polychaetes (Po- lydora websteri), and miscellaneous amphipods, isopods, and decapod crustaceans. In addition, during storage, sessile organisms had been in contact with the American eel, Anguilla rostrata, and the blue crab, Callinectes sapidus. Water from this mixed population was compared to a 1 ^m filtered sea water control. Next, a series of fi
Size: 1947px × 1283px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
