. A text-book of bacteriology. Bacteriology. 38 CULTURE MEDIA. the habit of collecting it in this way, and, after the serum has sepa- rated, of drawing it off in little flasks having a long neck, as shown in Fig. 14. The neck of the flask, previously steriUzed by heat, is shpped into the Woulf's bottle beside the cotton plug, the bulb (a) having been previously gently heated to expand the contained air. As the heated air cools a partial vacuum is formed and the clear mounts into the little flask. One after another is filled in serum this way, and each one is hermetically sealed in the flame of


. A text-book of bacteriology. Bacteriology. 38 CULTURE MEDIA. the habit of collecting it in this way, and, after the serum has sepa- rated, of drawing it off in little flasks having a long neck, as shown in Fig. 14. The neck of the flask, previously steriUzed by heat, is shpped into the Woulf's bottle beside the cotton plug, the bulb (a) having been previously gently heated to expand the contained air. As the heated air cools a partial vacuum is formed and the clear mounts into the little flask. One after another is filled in serum this way, and each one is hermetically sealed in the flame of a lamp a-. -a FiQ. 14. Fig. 15. Fio. 16. as soon as it is withdrawn. The sterile blood serum may be pre- served indefinitely in this way, and may be used as a liquid culture medium in the little flask, or it may be transferred to a test tube and solidified by heat whenever a solid blood-serum medium is re- quired. The advantage of preserving blood serum and other liquid media in these little flasks is in the fact that they may be preserved indeflnitely without becoming contaminated or drying up, and that they are easily transported, while a liquid medium in a test tube must be kept upright. The contents of one of these flasks are readily. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Sternberg, George Miller, 1838-1915. New York, W. Wood and company


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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbacteri, bookyear1901