. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COCKROACH SYMBIONT TRKHALASK 273 40 80 Log V 300 200 100 50. o — \ o 28 32 36 10 V (umoles hyd 3 mg prot hr'1) * 100 FIGURE 6. Arrhenius Plot ( 1/T rs. log K) of trehalase activity; incubation mixtures contained in a final volume of ml : 50 ^molcs citrate buffer pH , 20 /mioles trehalose and ml enzyme. 1961 ; Clegg and Filnsa, 1C'(>1 ) has increased the interest in this sugar. It is of considerable importance therefore to have a rapid accurate means of assaying this non-reducing disaccharide. \\e feel the t
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. COCKROACH SYMBIONT TRKHALASK 273 40 80 Log V 300 200 100 50. o — \ o 28 32 36 10 V (umoles hyd 3 mg prot hr'1) * 100 FIGURE 6. Arrhenius Plot ( 1/T rs. log K) of trehalase activity; incubation mixtures contained in a final volume of ml : 50 ^molcs citrate buffer pH , 20 /mioles trehalose and ml enzyme. 1961 ; Clegg and Filnsa, 1C'(>1 ) has increased the interest in this sugar. It is of considerable importance therefore to have a rapid accurate means of assaying this non-reducing disaccharide. \\e feel the trehalase isolated from the symbiont of Blabcnts represents a source for such an analytical enzyme. The trehalase reported herein has a pH optimum of which is somewhat lower than most of the enzymes heretofore reported, one exception being that of the trehalase from As pa-gill us oryzac reported to have optimum activity at pH (Horikoshi and Ikeda, 1966). Courtois, Petek and Kolahi-Zanovzi (1962), also reported that an enzyme from yeast possessed two optima, and at and one at , however, the data presented by those authors did not reveal well defined peaks of hydrolytic activity at either the above hydrogen ion concentrations. The pH optimum and Km of the trehalase from the symbiont of Blab ems craniijcr is quite similar to that reported by Gilby, \Vyatt and \Yyatt (1966) for the midgut trehalase of Blabcrns discoidalis. Those authors obtained a 100,000 g supernatant fraction from homogenates of washed gut tissue. \Yhether there was any relation-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, A
Size: 1658px × 1506px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
