. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 280 G. BAGHDASARIAN AND L. MUSCAT1NE incubated for h at 25°C in the light, and the other eight were incubated for h at °C in the light. After the incubation, algae in the incubation medium were recovered by centrifugation and set aside for analysis. Algae remain- ing in the animals were isolated by homogenization and centrifugation (Steen. 1987). Both the algae expelled into the medium and those retained by the hosts were counted, and the MI of the expelled algae was determined (Hoegh- Guldberg er al., 1987). M
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 280 G. BAGHDASARIAN AND L. MUSCAT1NE incubated for h at 25°C in the light, and the other eight were incubated for h at °C in the light. After the incubation, algae in the incubation medium were recovered by centrifugation and set aside for analysis. Algae remain- ing in the animals were isolated by homogenization and centrifugation (Steen. 1987). Both the algae expelled into the medium and those retained by the hosts were counted, and the MI of the expelled algae was determined (Hoegh- Guldberg er al., 1987). Mitotic index and rate of expulsion of algae in corals Mitotic indices and rates of algal expulsion were also estab- lished for four species of scleractinian corals commonly found in Kaneohe Bay, Oahu, Hawaii. Six pieces of each coral type from different colonies (P. compressa, M. verrucosa, P. dami- comis, and in the case of F. scutaria, entire corals) were incubated in 30 ml of FSW at 27°C (seawater temperature in Kaneohe Bay) for h. Next, to determine the effect of slight elevations of temperature on MI values and algal expulsion rates, six additional pieces from each coral type were incubated at °C for the same length of time. At the end of the incubation period, the corals were removed from the seawater. The released algae were collected by high-speed centrifugation of the incubation medium using a Damon IEC clinical centri- fuge, followed by resuspension in 5 ml of fresh FSW. Algae remaining in the corals were removed with a toothbrush. The product was collected in FSW. The algae were then cleaned of mucus and animal tissue by centrifugation and resuspension in 20 ml of FSW, and MI values and rates of algal expulsion were determined using the techniques described above. Results Mitotic index of algae: natural expulsion vs. mechanical isolation Algae naturally expelled from A. pulchella have a higher MI than the algae remaining in the hosts (Fig. 1; Wilcoxon si
Size: 1085px × 2304px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
