. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOLYMPH COAGULATION IN CRUSTACEA 63 1= H. FIGURE 3. Schematic representation of crossed immunoelectrophoresis of the hemolymph from Homarus vulgaris. H shows the hemocyanin and C represents the clottable factor. less completely with the fibrinogen of the NEM sample were observed in the PMSF H2O hemolymph (Fig. 5). DISCUSSION In lobsters and spiny lobsters, hemolymph coagulation includes morphological changes in amoebocytes, followed by a release reaction, a cellular aggregation, and a beginning of retraction. This cellular
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HEMOLYMPH COAGULATION IN CRUSTACEA 63 1= H. FIGURE 3. Schematic representation of crossed immunoelectrophoresis of the hemolymph from Homarus vulgaris. H shows the hemocyanin and C represents the clottable factor. less completely with the fibrinogen of the NEM sample were observed in the PMSF H2O hemolymph (Fig. 5). DISCUSSION In lobsters and spiny lobsters, hemolymph coagulation includes morphological changes in amoebocytes, followed by a release reaction, a cellular aggregation, and a beginning of retraction. This cellular transformation is rapidly hidden by the gelling of the whole plasma, which forms an "unretracted" clot. With inhibitors which delay whole-plasma clotting, but do not inhibit amoebocyte aggregation, complete retraction of the cellular clump is observed, recalling the thrombosthenin effect of vertebrate platelets. When plasma clotting is prevented, the "fibrinogen- like factor" always appears as a polymer scale in acrylamide gradient gels (min- imum four bands), but as a single precipitate line in crossed immunoelectrophoresis. This electrophoretic heterogeneity is in agreement with the ultracentrifugation analysis of Duchateau and Florkin (1954) and Stewart et al. (1966), who have found both 21 S and 17-18 S components in the blood of Homarus. Fuller and Doolittle (197la) have reported on the presence of S and S fibrinogen in Panulirus interruptus. Moreover, the latter authors have shown through disc electrophoresis that fibrinogen A is itself heterogeneous. With our specimens, DC situates these polymers from about 420,000 daltons (above 16 S hemocyanin) up to the top of the acrylamide gel. Fuller and Doolittle (197la, b) and Doolittle and Fuller (1972), indicate the same weight for the "monomer" (fibrinogen B) of Pan- ulirus. However, our "dimer" is not a multiple of 420,000, since the weight increase is of about 200,000 dalton
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
