. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PHOSPHATE UPTAKE BY EMBRYOS 135. \ cm. I"[(;i/KK 1. Perfusion chamber, c, cooling chamber; e, egg chamber; g, end-window Geiger- Mueller tube; i, inlet tube; o, outlet tube; p, platform; s, No. 1 coverslip. ° C.; however for several experiments, the temperature was lowered to ° C. Before each of these low temperature experiments, it was established with a thermo- couple inserted into the chamber that the temperature of the water flowing through the egg chamber was ° C. At the beginning of each experiment, the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PHOSPHATE UPTAKE BY EMBRYOS 135. \ cm. I"[(;i/KK 1. Perfusion chamber, c, cooling chamber; e, egg chamber; g, end-window Geiger- Mueller tube; i, inlet tube; o, outlet tube; p, platform; s, No. 1 coverslip. ° C.; however for several experiments, the temperature was lowered to ° C. Before each of these low temperature experiments, it was established with a thermo- couple inserted into the chamber that the temperature of the water flowing through the egg chamber was ° C. At the beginning of each experiment, the eggs were perfused with sea water for fifteen minutes, while a background count for the experiments was obtained. Sea water containing P32 was then turned on and the activity in the chamber reached a new level with the unfertilized eggs. Fifty to sixty minutes after the start of the experiment, the eggs were fertilized. This was accomplished by injecting to cc. of a 10% sperm suspension in P32-sea water into the chamber inlet tube with a hypodermic syringe. Fertilization and development of the eggs were observed with a Zeiss Opton stereoscopic microscope placed above the chamber. At the end of each experiment the eggs were removed from the chamber, washed, and allowed to develop further to check on their normality and on their recovery from the effects of any reagent that was being tested. After each experiment the chamber was rinsed alternately with concentrated HC1 and NaOH and perfused with tap water and sea water in order to remove P32 adsorbed on the inner surfaces of the bottom compartment. Any radioactivity left in the chamber was accounted for by measur- ing the activity of the empty chamber just before each experiment. In some experiments, eggs were activated parthenogenetically by the double treatment of Loeb (Just, 1939). The butyric acid and hypertonic sea water solutions were injected by means of a syringe into the egg chamber containing the unfertilized
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
