. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . varietycontained in the material, and if the oxygen access, moisture, and temperatureof incubation were favorable for the growth of all the varieties. This is animpossibility. Most nearly accurate results are obt^ned where only one or atmost several varieties are present and the conditions for their growth main-tained, Although the method gives an incorrect result when mixed materialis used, the enumeration of the colonies that develop is of a great deal of -practi-cal value, as ia tk e^^jMliatiiw
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . varietycontained in the material, and if the oxygen access, moisture, and temperatureof incubation were favorable for the growth of all the varieties. This is animpossibility. Most nearly accurate results are obt^ned where only one or atmost several varieties are present and the conditions for their growth main-tained, Although the method gives an incorrect result when mixed materialis used, the enumeration of the colonies that develop is of a great deal of -practi-cal value, as ia tk e^^jMliatiiw of loiDs and water. If the methods employed CULTIVATION OF MICROORGANISMS 115 are always the same, the comparison of the results gives us valuable informationas to the bacterial content. The method employed is in general as foUows: To 9 of water is added1 of the material to be examined; this is thoroughly shaken to separate theorganisms; 1 of this is then added to 9 of water thoroughly shaken,and 1 of this added to 9 of water and so on. This gives a dilution of.
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