. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SIBLING SPECIES OF BUGULA NERITINA 275 which is in Phase II clinical trials for the treatment of leukemias. lymphomas, melanomas, and solid tumors (Pluda ct til., 1996). Pettit and colleagues observed that bryostatin 1 was found in B. neritiiw samples from Califor- nia, but not from the Gulf of Mexico (Pettit. 1991). In more extensive work in Southern California. Mendola observed that bryostatin 1 is typically found in deeper samples, but is absent from shallow populations (D. Mendola. pers. comm.). We have defined two bryos
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SIBLING SPECIES OF BUGULA NERITINA 275 which is in Phase II clinical trials for the treatment of leukemias. lymphomas, melanomas, and solid tumors (Pluda ct til., 1996). Pettit and colleagues observed that bryostatin 1 was found in B. neritiiw samples from Califor- nia, but not from the Gulf of Mexico (Pettit. 1991). In more extensive work in Southern California. Mendola observed that bryostatin 1 is typically found in deeper samples, but is absent from shallow populations (D. Mendola. pers. comm.). We have defined two bryostatin chemotypes, O and M, on the basis of the observations of Pettit and col- leagues in California and Gulf of Mexico populations (Pet- tit. 1991, Fig. 1). ChemotypeO(octa-2,4-dienoate) contains bryostatins with an ester at C-20 (bryo- statins 1-3, 12. and 15) and small amounts of the minor bryostatins 4-9. The presence of bryostatins 1, 2, or 3 is diagnostic of chemotype O. Chemotype M (minor) contains the minor bryostatins 4-9 only, and lacks the octa-2,4- dienoate ester at C-20. The presence of minor bryostatins and the absence of bryostatins 1. 2, or 3 is diagnostic of chemotype M. Because the occurrence of bryostatins 10-18 is not well characterized, they are not included in the chemotype descriptions. To investigate the relationship between chemotype and genetic variation in B. neririna, three characters were ana- lyzed in samples from 13 locations at various depths along the California coast (Fig. 2). and from one Atlantic location (North Carolina): (1) bacterial symbiont small subunit ribo- somal RNA gene sequence (SSU rRNA). (2) host bryozoan mitochondria] cytochrome c oxidase subunit I gene se- quence (CO I), and (3) bryostatin content. Materials and Methods Sampling regime Colonies of B. neririiui were collected by hand, off docks or subtidally using scuba, along the California coast from Humboldt Bay to San Diego (Fig. 2). B. neririna was collect
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
