. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHICK SOMITE FORMATION 425 the containers of media were moistened with sulfadiazine ( per cent) as a further precaution against microbial contamination. RESULTS AND DISCUSSION The development of somites in DPS explants cultured on the media outlined above for 22 hours is given in Table II. One of the most striking observations is that the food materials used in this morphogenetic process are present free in the dialyzable (small molecule) FIGURE 2. Photograph of a DPS blastoderm cultured for 22 hours on dialyz
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHICK SOMITE FORMATION 425 the containers of media were moistened with sulfadiazine ( per cent) as a further precaution against microbial contamination. RESULTS AND DISCUSSION The development of somites in DPS explants cultured on the media outlined above for 22 hours is given in Table II. One of the most striking observations is that the food materials used in this morphogenetic process are present free in the dialyzable (small molecule) FIGURE 2. Photograph of a DPS blastoderm cultured for 22 hours on dialyzed whole yolk. Note absence of any appreciable development. FIGURE 3. Photograph of an embryo of similar age cultured for 22 hours on a medium containing yolk dialysate. Note the formation of somites. The same was found to be true when the egg white was assayed (Fraser, 1957). Somites developing in explants on the dialyzable fraction of whole yolk were equal in number to those in explants cultured on the yolk medium itself. On the other hand, no somites formed in blastoderms cultured on the large molecule moiety, even when glucose was added to the medium. This observation would indicate, in contrast to the conclusion of Taylor and Schechtman (1949), that the embryo in culture cannot use native protein alone from either yolk or albumen. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
