Blister canker of apple trees; a physiological and chemical study .. . m of fractions r-, ,.- jI and 2 tested Factions i and 2separately , combmed Healthy, after 23 , 38 . . . 64 3 . 06 The mechanism by wliich gelatine increases the oxidase activityis not clear. It is evidently not through buffer action, as shown byits lack of effect on the hydrogen ion concentration (table XVII,figs. 8, 9, 10). Special tests showed that there was no hydrolysisof the gelatine to amino acids, in either healthy or diseased bark,which would increase its buffer effect.
Blister canker of apple trees; a physiological and chemical study .. . m of fractions r-, ,.- jI and 2 tested Factions i and 2separately , combmed Healthy, after 23 , 38 . . . 64 3 . 06 The mechanism by wliich gelatine increases the oxidase activityis not clear. It is evidently not through buffer action, as shown byits lack of effect on the hydrogen ion concentration (table XVII,figs. 8, 9, 10). Special tests showed that there was no hydrolysisof the gelatine to amino acids, in either healthy or diseased bark,which would increase its buffer effect. If gelatine is effectivethrough its action as a protective colloid, its effect in this directionmust be very complex, as shown by its difference in effect on barkmixtures and precipitated oxidases. I30 BOTANICAL GAZETTE FEBRUARY 0) . J» €| ^?^ 1 ft) 1 1 ziiz:^^^^ —? -^Z- _=ti—^=^ B 7/me /n hours Fig. 8.—Oxidation of pyrocatechin by first and second fractions of healthy bark,with gelatine (for explanation of lettering see legend for fig. 7).. 12 3 16 40 Fig. 9.—Oxidation of pyrocatechin by first and second fractions from diseasedbark, without gelatine (for explanation of lettering see legend for fig. 7); points ofplotting marked by vertical broken lines.
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Keywords: ., bookcentury1900, bookdecade1910, bookpublisherchica, bookyear1919