Collected papers . -5 0-0636 7-482 86 30 0-1809 6-030 165 21 0-1025 4-881 5-53 10 12-7 0-1201 9-458 30 15-5 0-1279 8-251 60 16 0-1144 7151 120 18-5 0-1058 5-719 241 18-7 0-0802 4-288 420 14-8 0-0428 2-892 600 36 0-0709 1-969 4-01 10 13 0-1230 9-461 40 12-5 0-1000 8-001 90 16 0-1073 6-707 215 17-5 0-0836 4-777 300 20 0-0810 4-050 645 24 0-0589 2-454 4-81 See Table III, Experiment at 69° C. 420 H. CHICK AND C. J. MARTIN. of alkalinity possible without formation of alkali albumen^ (Exp. 7).This is after all a very small range, but was the utmost within thecompass of our experimental method for an


Collected papers . -5 0-0636 7-482 86 30 0-1809 6-030 165 21 0-1025 4-881 5-53 10 12-7 0-1201 9-458 30 15-5 0-1279 8-251 60 16 0-1144 7151 120 18-5 0-1058 5-719 241 18-7 0-0802 4-288 420 14-8 0-0428 2-892 600 36 0-0709 1-969 4-01 10 13 0-1230 9-461 40 12-5 0-1000 8-001 90 16 0-1073 6-707 215 17-5 0-0836 4-777 300 20 0-0810 4-050 645 24 0-0589 2-454 4-81 See Table III, Experiment at 69° C. 420 H. CHICK AND C. J. MARTIN. of alkalinity possible without formation of alkali albumen^ (Exp. 7).This is after all a very small range, but was the utmost within thecompass of our experimental method for any one temperature. As itwas, the reaction rate in the most acid condition (Exp. 1) was so rapidas to make the observations only just possible, whereas the exjperimentwith neutral solution occupied 12 hours. The results are shown graphically in Fig. 5 where the concentrationof residual albumen in solution for seven different degrees of acidity areplotted against time. The observations fall upon smooth curves. As in. 50 100 T/Aff IN M/NUTCS Fig. 5. Influence of acid upon coagulation rate at 69° C. of egg-albumen in 1 ^[^ solutionsto which varying amounts of alkali had been added. Ordinates = concentration ofalbumen in milligrams per AbscissaB = Time minutes. 1. Original solution, reaction acid, H+ ion concentration greater than distilled water. N 1-6 ^ AmOH added per gram protein, reaction acid, «^ ^^ 5) >» >l )> 4- ^2 ,, }) )» «) 5. 35o ,, ,, ,, 6. 401 reaction alkaline, H+ ion concentrationless than distilled water. 7. 4-81 i ^ It must be remembered that the original solution of twice re-crystallised egg-albumencrystals is decidedly acid and that we have studied the effect of varying the acidity byadding different amounts of alkali. This method was employed owing to the fact that atthat time we had no means of ascertaining the true neutral point of the solution. It hasno dipadvantage other than rendering the description involved. HEAT COAGULAT


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