. Bacteriology in medicine and surgery. A practical manual for physicians, health officers, and students. Bacteriology. BA CTEBIOL 0 QICAL TEGHNIQ UE. 223 cent, carbolic acid solution for at least thirty minutes and then rinsed again with sterile water. New tubes and flasks sometimes require to be washed in a 2 per cent, solution of nitric acid, so as to remove any free alkali which may be present. They are finally thor- oughly rinsed in pure water. Old tubes, flasks, and other glassware are boiled for about thirty minutes in a 5 per cent, solution of washing soda and then thoroughly rinsed of
. Bacteriology in medicine and surgery. A practical manual for physicians, health officers, and students. Bacteriology. BA CTEBIOL 0 QICAL TEGHNIQ UE. 223 cent, carbolic acid solution for at least thirty minutes and then rinsed again with sterile water. New tubes and flasks sometimes require to be washed in a 2 per cent, solution of nitric acid, so as to remove any free alkali which may be present. They are finally thor- oughly rinsed in pure water. Old tubes, flasks, and other glassware are boiled for about thirty minutes in a 5 per cent, solution of washing soda and then thoroughly rinsed off with water until perfectly clean. If neces- FlQ. Dry heat sterilizer. sary, any dirt clinging to the insides of the flasks and tubes can be removed by bristle brushes or suit- able swabs. After the tubes and flasks have been thoroughly cleaned they are plugged loosely with ordi- nary cotton batting, or, if that is not at hand, the more expensive absorbent cotton. The tubes and flasks with their cotton plugs and all other glassware are sterilized by dry heat at 150° C. for one hour (Fig. 26). The sterile tubes and flasks are filled with the media, when small quantities are used, by means of a glass funnel. The main precaution to be observed is not to let the media soil the neck of the tubes and flasks, as. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Park, William Hallock, 1863-1939; Guerard, Arthur Rose, 1851-. New York and Philadelphia, Lea Brothers & Co
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