. Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . Fig. 2. Golgi apparatus from a normal animal. One Golgi unit with several membranes, a tew large vacuoles and some small vesicles. Magnification 53,000. Table 1. The vacuolization of the Golgi apparatus after Diamox. Number Experiment Number of Golgi Number of vacu- of regions oles per Golgi 'mals observed region per section per cell ani Normal animals; no 12 nephrectomy Control animals after 3 nephrectomy (Diazil) 30 min. after 10 mg 3 Diamox per kg body weight; nephrectomy 60 min. after 10 mg 3 Diamox per kg bo
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . Fig. 2. Golgi apparatus from a normal animal. One Golgi unit with several membranes, a tew large vacuoles and some small vesicles. Magnification 53,000. Table 1. The vacuolization of the Golgi apparatus after Diamox. Number Experiment Number of Golgi Number of vacu- of regions oles per Golgi 'mals observed region per section per cell ani Normal animals; no 12 nephrectomy Control animals after 3 nephrectomy (Diazil) 30 min. after 10 mg 3 Diamox per kg body weight; nephrectomy 60 min. after 10 mg 3 Diamox per kg body weight; nephrectomy 120 min. after 10 mg 3 Diamox per kg body weight 30 min. after 100 mg 3 Diamox per kg body weight; no nephrectomy 31 78±10 7 79 + 11 4 86±18 7 93±12 13 193-12 11 173 â 11 4 228 z 40 6 184+13 4 142+ 7 5 155-15 6 79 ⢠10 6 116:* 15 9 118r 7 6 195r23 6 174+16 4 198 + 25 86 r 4 198±16 160±12 104 â i: 189: now, there are no other changes in the mitochondria, but the analysis is not yet finished. In the Golgi apparatus there is a great increase in the amount of the small vesicles (fig. 3). A quantita- tive analysis has been performed by counting all vesicles seen in the Golgi apparatus on one section through a cell. The result is presented in Table 1. This informs of the fact that the changes in the Golgi apparatus appear very soon and that the number of vesicles decreases in animals killed at longer intervals after the injection of Diamox. Thus, after two hours the amount of vesicles is about the same as in the control animals. Another change in the Golgi apparatus concerns the Golgi membranes, which normallv lie in 2-3 rows with 4-5 membrane pairs in each, sometimes split up into several units with onl\ 1 3 mem- brane pairs (fig. 3). Finally it must be pointed out that in the control animals no such variations have been observed in the cytoplasm, the mitochondria or in the Golgi appara- tus. Discussion.âConcerning the cell structure under
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