. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 78 JOXATHAX 1'. Hole, Massachusetts. The crabs were maintained unfed in a fiberglass tub with perforated floor. Flowing sea water maintained a water level of about i inch. Crabs were utilized randomly from this supply. Entire and destalked animals were injected with ml of 2, 5, -:;l l-tyrosine in sea water through the arthrodial membrane at the base of the fourth walking leg. All crabs were dechelated to reduce the hemocoel volume, and maintained for two days on a black background to maximize pigment dis
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 78 JOXATHAX 1'. Hole, Massachusetts. The crabs were maintained unfed in a fiberglass tub with perforated floor. Flowing sea water maintained a water level of about i inch. Crabs were utilized randomly from this supply. Entire and destalked animals were injected with ml of 2, 5, -:;l l-tyrosine in sea water through the arthrodial membrane at the base of the fourth walking leg. All crabs were dechelated to reduce the hemocoel volume, and maintained for two days on a black background to maximize pigment dispersal. The tissue chosen for examination was the epidermis lining the carapace. This tissue is extremely friable and cannot be stripped from the exoskeleton without considerable destruction and distortion. Consequently the epidermis-exoskeleton complex was fixed /;/ situ in San Felice fixative ( 10r/ formalin in sea water con- taining \% chromic acid plus 10 drops acetic acid). After fixation the hardened epidermis was stripped from the exoskeleton with minimal derangement of the tissue. The epidermis was washed in several changes of distilled water to remove the fixative and free tyrosine. The epidermis was then spread on gelatin-coated microscope slides (Brunet and Small, 1959). Slides were dipped in Kodak NTB-2 liquid photographic emulsion diluted ^ with distilled water, dried and stored in light-tight boxes in the refrigerator for 35 days. They were developed in Dektol. fixed, washed and *. FIGURE 1. Autoradiograph prepared from a whole-mount of fiddler crab epidermis with dispersed pigment; bar = 50 /x; phase contrast optics. FIGURE 2. Autoradiograph prepared from a whole-mount of fiddler crab epidermis ; detail of Figure 1, bar = 50 AI; phase contrast Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
