. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 92 E. NEWTON HARVEY. gas. I believe the addition of platinized asbestos to the fluid through which hydrogen is passed will prove of great value to biologists in the study of cells under anaerobic conditions. Instead of Pt asbestos, colloidal Pt or colloidal Pd may be used. The latter is conveniently prepared by adding to 5 cc. per cent. PdCl2 just enough dilute NaOH to make the PdCl2 darken without giving a precipitate of basic PdCl2, and then one drop of .042 per cent, hydrazine hydrate. The finely divided Pd adsorbs a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 92 E. NEWTON HARVEY. gas. I believe the addition of platinized asbestos to the fluid through which hydrogen is passed will prove of great value to biologists in the study of cells under anaerobic conditions. Instead of Pt asbestos, colloidal Pt or colloidal Pd may be used. The latter is conveniently prepared by adding to 5 cc. per cent. PdCl2 just enough dilute NaOH to make the PdCl2 darken without giving a precipitate of basic PdCl2, and then one drop of .042 per cent, hydrazine hydrate. The finely divided Pd adsorbs a good deal of hydrogen and will keep a solution free of oxygen for some time, as can be observed by using as indicator the luminescence of a mixture of luciferin and luciferase. In order to show that the luminescence of Ctenophores, like Beroe, is independent of dissolved oxygen, it must be recalled that an extract made by squeezing Beroe through muslin and filtering through filter paper is non-luminescent itself, but gives a brilliant light on addition of fresh water, dilute salt solution, or various cytolytic substances like saponin, sodium glycocholate, ether, etc. Accordingly I tried the experiment of mixing Beroe extract made free of oxygen by Na2S2O4 with m/io Na2HPO4 solution, also made free of oxygen by Na2S2O4. The Na2HPO4 was used instead of fresh water in order that the reaction would be alkaline enough to allow rapid coloration of leuco-methylene blue, added as an indicator, should any oxygen leak into the apparatus. This is shown in Fig. i. In A is placed Beroe extract + Na2S2O4. FIG. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947;
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
