. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 432 MARIA DOLORES PEREZ-GONZALEZ solutions to which an equal part or a fourth part of isotonic sucrose is added, the granules remain largely intact. The activity of the hormone in these media (Fig. 5) is comparable to that in isotonic sucrose. In another group of experiments an effort was made to find the best medium for preservation of the granules. Watanabe and Williams (1953) have shown that bovine plasma albumin in isotonic potassium phosphate buffer at pH 7 is a good medium to preserve mitochondria of insect muscle
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 432 MARIA DOLORES PEREZ-GONZALEZ solutions to which an equal part or a fourth part of isotonic sucrose is added, the granules remain largely intact. The activity of the hormone in these media (Fig. 5) is comparable to that in isotonic sucrose. In another group of experiments an effort was made to find the best medium for preservation of the granules. Watanabe and Williams (1953) have shown that bovine plasma albumin in isotonic potassium phosphate buffer at pH 7 is a good medium to preserve mitochondria of insect muscles. In the following experi- ments, besides the M sucrose, a mixture of 25% M sucrose and 75% sea water was also used, as well as bovine plasma albumin in M potassium Oty LU o: I2 a: o. 10 20 30 40 50 60 TIME IN MINUTES FIGURE 5. Response of black chromatophores of Uca to injections of homogenates of sinus glands in different solutions: O, distilled water; f>, sea water; O, and M NaCl; o, M KC1; e, G, and 3, NaCl, KC1 and sea water in a mixture with 25% of M sucrose. phosphate at pH 7. The homogenates of sinus glands in these three media were kept at 2° C. and at different times were diluted in M sucrose and assayed using legs of Uca (Fig. 6). After 6 hours of incubation in these media, the activity of the black chromatophore-dispersing hormone is insignificant, and after 24 hours only a slight effect was observed. That the hormone was preserved in the granules was shown by the following procedure. After 24 hours the homogenates were heated for 5 minutes in boiling water and diluted in sea water. After this treat- ment all the solutions produced a maximal dispersion of the chromatophores, com- parable to that caused by homogenates in distilled water. Thus, the three different media used seem to be equally efficient in keeping the granules Please note that these images are extracted from scanned page images that may have been
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