. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 16 BACTEKIOLOGY the purpose of regulating the temperature. This arrange- ment is suited for the sterilisation of instruments, apparatus, glassware, &c. (fig. 9). To prevent flasks and test-glasses from becoming re- infected after sterilisation they must be closed with a plug of cotton-wool before being placed in the steriliser, as such a plug, while allowing the air to enter the vessels, keeps back the organisms floating in it. The plug can b$ further covered with a cap of
. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. 16 BACTEKIOLOGY the purpose of regulating the temperature. This arrange- ment is suited for the sterilisation of instruments, apparatus, glassware, &c. (fig. 9). To prevent flasks and test-glasses from becoming re- infected after sterilisation they must be closed with a plug of cotton-wool before being placed in the steriliser, as such a plug, while allowing the air to enter the vessels, keeps back the organisms floating in it. The plug can b$ further covered with a cap of indiarubber. Instead of plugging with cotton, Stafl'-surgeon Schill re- commends the use of double test-glasses, consisting of two test-tubes made of stout glass and with smooth even edges, one 1 Ml'^imHli "^ which is pushed over the i I fchi other as a cover. The for- m^- I Ifc^..^ mer should be only so much _^ I . ...,^^^- wider as to leave a space the thickness of a sheet of paper Fig. 9.—Ho'i'-AiR Steriliser. ^ ^ between the two, and should be but half as long as the lower. Sterilisation by steam.—Articles which cannot be ex- posed to so high a temperature are sterilised by the vapour of boiling water at 100° C. For this purpose a cylindrical vessel of sheet copper is used, measuring nearly a metre in height and about 20 cm. in diameter, covered with felt or asbestos to prevent loss of heat, and capable of being closed with a lid similarly protected. The latter is provided with an opening for the introduction of a ther- mometer, and does not fit quite air-tight (fig. 10). The bottom of the vessel is double, the inner bottom consisting of a grating fixed about 30 cm. above the outer, and the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Schenk, Samuel Leopold, 1840-1902. London, Longmans, Gre
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