. Biological structure and function; proceedings. Biochemistry; Cytology. 232 MARTIN KLINGENBERG TABLE II Redox State of Pyridine Nucleotides in Skeletal Muscle Mitochondria Additions DPNH / TPNH / DPNH ^DPN* Glycerol-1-P Glycerol-1-P + ATP Succinate Succinate + ATP Pyruvate + malate 0-72 2-05 030 1-85 I-So 0-47 0-58 0-39 o-6o o-SS o-17 0-47 o-o8 0-44 032 Mitochondria after washing with serum albumin Glycerol-1-P 2-02 044 042 Succinate 3* 01 0-46 063 Pyruvate + malate 2-28 0'44 0-48 Incubated in 03 M sucrose, 10 mM triethanolamine-HCl-bufFer, i mM EDTA, pH 72, 25°. Conc
. Biological structure and function; proceedings. Biochemistry; Cytology. 232 MARTIN KLINGENBERG TABLE II Redox State of Pyridine Nucleotides in Skeletal Muscle Mitochondria Additions DPNH / TPNH / DPNH ^DPN* Glycerol-1-P Glycerol-1-P + ATP Succinate Succinate + ATP Pyruvate + malate 0-72 2-05 030 1-85 I-So 0-47 0-58 0-39 o-6o o-SS o-17 0-47 o-o8 0-44 032 Mitochondria after washing with serum albumin Glycerol-1-P 2-02 044 042 Succinate 3* 01 0-46 063 Pyruvate + malate 2-28 0'44 0-48 Incubated in 03 M sucrose, 10 mM triethanolamine-HCl-bufFer, i mM EDTA, pH 72, 25°. Concentration of substrates: 4 mM; ATP: i mM. *i;DPN = DPN + DPNH. isolated from some other organs. The second part of Table II shows that, after washing the skeletal-muscle mitochondria with serum albu- min, no ATP is required for DPN reduction. When added after the sub- strates, albumin is also effective in facilitating the DPN reduction, as shown in Fig. 3. It is assumed that albumin reverses an endogenous. DPN reduction Fig. 3. The effect of serum albumin, antimycin A and ATP on the redox state of the mitochondrial DPN in the presence of glycerol-1-phosphate. The absorption trace (dashed curve) is corrected for the absorption due to albumin and by a shift of the pen position, for the absorption due to antimycin A (cf. legend Fig. 2.).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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