. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 2X2 P. C. ROTHLISBERG ET 'T' I ~T I 1 i 1 1 1 1 E 1 1 1 1 l 1 1 1 1 1 1 i | ^J 1 ^ FIGURE 1. Shipboard larval rearing rack (B). with three water baths (1,2. 3) for larval rearing, one (4) for rearing brine shrimp and storage (5) for spare larval rearing beakers. Female isolation chambers (E) in circulating water baths (D). Pump (C) to supply surface sea water for temperature regulation of both the larval incubation trays and the female holding containers. Two aerators (A) to supply air to individual larval and adult con
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 2X2 P. C. ROTHLISBERG ET 'T' I ~T I 1 i 1 1 1 1 E 1 1 1 1 l 1 1 1 1 1 1 i | ^J 1 ^ FIGURE 1. Shipboard larval rearing rack (B). with three water baths (1,2. 3) for larval rearing, one (4) for rearing brine shrimp and storage (5) for spare larval rearing beakers. Female isolation chambers (E) in circulating water baths (D). Pump (C) to supply surface sea water for temperature regulation of both the larval incubation trays and the female holding containers. Two aerators (A) to supply air to individual larval and adult containers. See text for details of construction and operation. siosira pseudonana, Isochrysis galbana, Tetraselmis (= Platymonas) suecica) that previously had been grown, harvested, concentrated to predetermined cell concen- trations, and frozen with dimethyl sulfoxide (DMSO) as a freeze-thaw protectant (Brown, 1972) in 10 ml plastic test tubes (Pendrey, unpublished). The algae were thawed and diluted to pre-determined feeding aliquots, on board, and fed to the larval cultures four times per day. Brine shrimp cultures were maintained on board for feeding to the older larvae (mysis and postlarvae) along with the algal diet. The sides of the rack were covered by 70% shade cloth to keep the cultures cool and prevent direct sunlight entering the larval containers. The top of the rack had a pitched waterproof canvas roof to protect the aerators from rain and salt spray. Female-holding and spawning containers Two large fiberglass tanks (500 X 1200 X 600 mm) (Fig. 1;D) with flow-through sea water circulation were used as water baths for maintaining gravid females at surface sea water temperatures. The females were held in 9 1 polyethylene bags (Fig. 1;E) (10 bags per water bath) held open with a polyethylene ring and suspended from a metal rack across the top of the water bath. A separate aquarium aerator was used for these female holding tanks. Airflow to each plastic bag was regulated
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
