. Pathological technique; a practical manual for workers in pathological histology and bacteriology. ail to prevent his es-caping through the other end. The animal is thus securedand ready for the operation of inoculation. A more com-plete form of this apparatus, with a fixed clamp for the ani-mals tail, is shown in Fig. 27. In making the inoculation the mouse is pulled backwardby the tail until his rump is exposed in the end of the cylin-der, and then with small scissors the hair is cut away over aspace, approximately 1 cm. square, about the root of thetail. In the center of this a small open
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. ail to prevent his es-caping through the other end. The animal is thus securedand ready for the operation of inoculation. A more com-plete form of this apparatus, with a fixed clamp for the ani-mals tail, is shown in Fig. 27. In making the inoculation the mouse is pulled backwardby the tail until his rump is exposed in the end of the cylin-der, and then with small scissors the hair is cut away over aspace, approximately 1 cm. square, about the root of thetail. In the center of this a small opening is made throughthe skin 3 or 4 mm. long with small scissors, and throughthe opening the points of the scissors are passed anteriorlybeneath the skin for a distance of about 1 cm., so as to makea pocket or cavity by separating the skin from the mus-cles. Into the cavity thus formed the material for inocula-tion is then to be introduced by means of the platinum a rule, white mice are to be preferred to the wild brownvariety, on account of the greater ease with which they maybe Fig. 27.—Mouse-holder, with mouse in position for inoculation. Mice may also be inoculated in the peritoneal cavity by intro-ducing a very few drops of a suspension or a bouillon cultureof an organism with a hypodermic syringe. The quantity of bacteria used for purposes of inoculationvaries with the organism and with the end in view. In gen-eral, it may be said that in inoculating with the growth from CULTURE METHODS. 233 a solid medium with the platinum wire one or two loopsfulare used. If bouillon cultures are employed, the quantityinjected varies from -^ to 1 in most cases. In cases where a suspension of the growth on a solidmedium is injected the same quantities are used as in thecase of bouillon cultures, the density of the suspension de-pending upon the operator. A suspension may be con-veniently prepared by pouring 5 or 8 of sterile bouillon,sterilized water, or
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Keywords: ., bookcentury1900, bookdecade1910, bookidpath, booksubjectpathology
