. Bulletin. Natural history; Natural history. 458 Illinois Natural History Survey Bulletin Vol. 26. Art. b (T 60 UJ UJ S 50 40 O 30 o UJ ^ 20 q: u ^ 10 Isolate 47-3. 10 15 20 25 30 TEMPERATURE, DEGREES CENTIGRADE Fig. 8.—Effect of temperature on the colony size of two basal dry rot isolates of Fusarium grown 160 hours on Coons's agar. several days. Five of the six isolates grew when moved to the lower temperature. Basal dry rot isolate 47-3 apparently had been killed by the 160-hour exposure to 40 degrees C. In these studies the general shape of the curves of growth responses of all isolates t
. Bulletin. Natural history; Natural history. 458 Illinois Natural History Survey Bulletin Vol. 26. Art. b (T 60 UJ UJ S 50 40 O 30 o UJ ^ 20 q: u ^ 10 Isolate 47-3. 10 15 20 25 30 TEMPERATURE, DEGREES CENTIGRADE Fig. 8.—Effect of temperature on the colony size of two basal dry rot isolates of Fusarium grown 160 hours on Coons's agar. several days. Five of the six isolates grew when moved to the lower temperature. Basal dry rot isolate 47-3 apparently had been killed by the 160-hour exposure to 40 degrees C. In these studies the general shape of the curves of growth responses of all isolates to various temperatures was of the same general pattern, figs. 6, 7, and 8. The basal dry rot isolates agreed much more closely in their responses to temperatures than did the isolates from the other disease forms. The vascular isolates showed the greatest divergence in responses to temper- atures. Isolates from the three disease forms could not be distinguished by their growth responses to various temperatures. Reactions to Aniline Dyes The 40 isolates of the gladiolus Fusar- ium listed in table 1 were grown on Coons's agar containing various concentra- tions of the aniline d\es malachite green, brilliant green, and crystal violet, accord- ing to the method developed bv Coons &; Strong (1931). The malachite green and brilliant green media were made from preparations of stock agar containing 10 milliliters of per cent dje solution per liter of Coons's agar. For each medium, four concentra- tions, tables 3 and 5, were prepared from the stock agar diluted with appropriate amounts of sterile, melted Coons's agar. The crystal violet stock agar was pre- pared from 100 milliliters of per cent dye solution added to a liter of Coons's agar, and the four concentrations, table 4, were made from this stock. Inoculum was prepared in the manner described for the temperature studies. Four isolates were grown in each dish. The center of each quadrant of the dish was seeded with a
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