. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 198 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS •40 i stationary self referencing mode. 200 300 time (s) 40 - 30 - $ 20- 3 10 0 -10 B 10 |jM rotenone 11 mM glucose 0 100 200 300 400 500 600 time (s) 30 o -" 10 10 nM forskolin 11 mM glucose 100 200 time (s) 300 400 Figure 1. I A) Real time trace (DO unit lonView processed trace (AC) of oxygen current as a function of distance from an oxygen source (diam- eter - 10 /nw) measured with a stationary and a self-referencing oxygen microsensor. In the self-referencing mode
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 198 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS •40 i stationary self referencing mode. 200 300 time (s) 40 - 30 - $ 20- 3 10 0 -10 B 10 |jM rotenone 11 mM glucose 0 100 200 300 400 500 600 time (s) 30 o -" 10 10 nM forskolin 11 mM glucose 100 200 time (s) 300 400 Figure 1. I A) Real time trace (DO unit lonView processed trace (AC) of oxygen current as a function of distance from an oxygen source (diam- eter - 10 /nw) measured with a stationary and a self-referencing oxygen microsensor. In the self-referencing mode, the oxygen sensor was oscil- lated within a Ill-pun i:\cnrsion a! a frequency of Hz. The numbers are the relative distance from the oxygen source in microns. (B) Influence o) rotenone on oxygen consumption by a single pancreatic HIT cell. The mice is a representative of five measurements. (C) Influence of forsknlin on oxygen consumption hy a single pancreatic HIT cell. The trace is a representative of four measurements. There is a significant difference in oxygen consumption after forskolin treatment (P < ). In the above single cell measurements, the oxygen sensor was positioned 10 from the plasma membrane of the cell and oscillated between 10 and 40 n/n ui 30-fjjn excursion! at a frequency of Hz. A potential of -600 mV vs. Ag/AgCI iri/.s applied to the o\\gen sensor in a modified HEPES buffer at 37°C for all experiments. cell/min (P < according to two-tailed Student's / tests). This decrease ( — 88%) is presumably due to the inhibition of NADH-dependent respiration. We injected forskolin, a specific activator of adenylate cyclase, to elevate cAMP level and observed a flux of ± prnol/cnr/s (mean ± SEM, n =: 4), u consumption of fmol/cell/min (see Fig. 1C). The oxygen con- sumption after forskolin treatment was significantly higher than before (P < ). This increase (-19%) may account for the ATP demand in insulin secreti
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
