. The microscope; an introduction to microscopic methods and to histology. Microscopes. CH. VII•] PARAFFIN SECTIONING 183 ? 2S1. Mounting in Balsam.—After the sections are stained they must be dehydrated and cleared before mounting in balsam. For the dehydration the slide is plunged into a jar of 95",, alcohol. For clearing after the dehydration the slide is drained of alcohol and put down flat and the clearer poured on, or the whole slide is immersed in a jar of clearer f§ 318). Clearing usually is sufficient in a few minutes ; a stay of an hour or even over night does not injure most se
. The microscope; an introduction to microscopic methods and to histology. Microscopes. CH. VII•] PARAFFIN SECTIONING 183 ? 2S1. Mounting in Balsam.—After the sections are stained they must be dehydrated and cleared before mounting in balsam. For the dehydration the slide is plunged into a jar of 95",, alcohol. For clearing after the dehydration the slide is drained of alcohol and put down flat and the clearer poured on, or the whole slide is immersed in a jar of clearer f§ 318). Clearing usually is sufficient in a few minutes ; a stay of an hour or even over night does not injure most sections. In mounting in balsam the clearer is drained away by standing the slide nearly vertically on some blotting paper, or by using the waste bowl and standing it up in the little funnel (Fig. 14S). Then the balsam is put on the sections or spread on the cover-glass and that placed over the sections. For cataloging and labeling, see yi. 307-310. Fig. 153. Small spirit lamp modified into a bal- sam bottle, or a glycerin or glycerin-jelly bottle, or a bottle for homogeneous immersion liquid. For all of these purposes it should contain a glass rod. See also Fig-. .§ 2S2. The Collodion Method with Alcohol.—A good method of procedure for making collodion sections is to do exactly as described, including ? 272, and then instead of hardening the collodion in chloroform and clarifier, it is hardened in S2"0 alcohol for a day or two before sectioning. In sectioning the knife and tissue are kept wet with 82% alcohol and the sections are dehydrated with 95% alcohol and then fastened to the slide with ether alone or with ether-alcohol. The staining and mounting {\ 27S-2S1 ) are as described. One may preserve the tissue after imbedding for a long time in the 82% alcohol before sectioning and sections may be made at any time. While this method appears somewhat simpler, the results are not so satisfactory as by the oil method given above. THE PARAFFIN METHOD § 2S3. As wi
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