. Carnegie Institution of Washington publication. 16 ON THE DIFFERENTIAL REACTION TO VITAL DYES EXHIBITED BY Protocol: Mouse 36, October 26, injected subcutaneously with c. c. of a 1 per cent solution of isamine blue 6B, a sulphonated triphenyhnethane dye, with the formula Nq035 ojX)jxrc&*. x—exact position of sulpbonic radical uncertain. November 3: Ears are pale blue; skin over back and sides is deep blue; subcutaneous tissue definite pale blue on side opposite injection-point. Injection-site contains many macrophages with typical form and deposits of varying sizes. Fibroblasts arc i
. Carnegie Institution of Washington publication. 16 ON THE DIFFERENTIAL REACTION TO VITAL DYES EXHIBITED BY Protocol: Mouse 36, October 26, injected subcutaneously with c. c. of a 1 per cent solution of isamine blue 6B, a sulphonated triphenyhnethane dye, with the formula Nq035 ojX)jxrc&*. x—exact position of sulpbonic radical uncertain. November 3: Ears are pale blue; skin over back and sides is deep blue; subcutaneous tissue definite pale blue on side opposite injection-point. Injection-site contains many macrophages with typical form and deposits of varying sizes. Fibroblasts arc in the majority well filled with large dye vacuoles, though these same cells may show processes quite free from dye as well as many small vacuoles in parts. In less heavily stained parts (transition zone is broad and vague), the cell types are more distinct, though many fibroblasts have large deposits. Here one finds rounded-up macrophages wit h immense vacuoles, while others are packed with paler, comparatively small deposits. The latter, however, are found containing one or many of the larger vacuoles, and similarly those with the largest vacuoles have some smaller also. However, such neighboring cells may be strikingly dissimilar in general appearance. The palely stained area shows macrophages with a moderate amount of dye, in the form of small vacuoles, some of which are colorless. The fibroblasts are seen, even under the oil, with more difficulty. They contain often a half dozen or more small round dye granules unrelated to the mitochondrial threads. Protocol: Rat 25-1, injected intraperitoncally with a per cent solution of the deep-blue " negative" dye H 7, made by combining 1 molecule of dianisidine with 2 molecules of alpha naphthol monosulphonic acid 4, and hence with the formula. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations ma
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