. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MUSSEL CYSTINE-RICH DOPA PROTEINS 133 n •e § 123456 r 60 £ c o 8 Elulion time (min). 80 g^ 60 B C O 40 8 0 20 40 60 80 Elution time (min) Figure 5. HPLC chromatography profiles of Mefp-2 and derivatives following proteolytic digestion with trypsin. Peptides were resolved on a X 25 cm C,g column using acetonitnle gradients as indicated by the dashed lines. Fractions of 1 ml were collected at a flow rate of 1 ml/ min. (A) Peptides and undigested core protein (fractions 68-72) derived from native Mefp-2. Inset: AU-PAGE ana
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MUSSEL CYSTINE-RICH DOPA PROTEINS 133 n •e § 123456 r 60 £ c o 8 Elulion time (min). 80 g^ 60 B C O 40 8 0 20 40 60 80 Elution time (min) Figure 5. HPLC chromatography profiles of Mefp-2 and derivatives following proteolytic digestion with trypsin. Peptides were resolved on a X 25 cm C,g column using acetonitnle gradients as indicated by the dashed lines. Fractions of 1 ml were collected at a flow rate of 1 ml/ min. (A) Peptides and undigested core protein (fractions 68-72) derived from native Mefp-2. Inset: AU-PAGE analysis of native Mefp-2. CAMC- Mefp-2 and COMC-Mefp-2 before (lanes 1-3, respectively), and after (lanes 4-6, respectively) proteolysis with a trypsin:protein weight ratio of 1:50. or 1:25 in the case of native Mefp-2. Equivalent concentrations of protein were run in each lane, and the gel was stained with Coomassie Blue. (B) Peptides derived from COMC-Mefp-2. The peptide map derived from CAMC-Mefp-2 was qualitatively similar to that shown in (B). Consensus (Type /): Peptide # Cystine Enriched Tryptic Peptides Oligopeptide Submotifs 1 234 56 (CX1CVGGYS3 PTC QEJC£ KPNPC [X]K XL GV V A COMC CAMC Sequence Type I peptides 38 32b CAPLSK 50b VNVC 64b 53b XIGAPNPQ K 57a 46b YVCAPNPCK 48a C GENVC KPNPC OK V A 49a C QVHAC KPNPC N{N/T)K 52a C GVNflCNPNPCK 62a 51b GGYSG PTC GENVC APNPC (£K 63a 52b GGYS; PTC GVNAC KPNPC ®R F 63b 51a CS GGYS; PTC NVNAC KPNP- N< 64a 53a CV PTC QENACKPNPS K 66a CTffV GGY33 PTC QEUflC KPNPC SN. . 69a CL GGYTG PTC -ED— KPNPC N. . 68 CL GGjnC PTC -ED— KPNPC NIK V NV 54b CYCVGGY33 FQ- . 71 CXCTGGYCG 58 54a £YfD N23DG PTC QEUAC KPNPC N. . 55b 52b CYTO K 31 27/28 CYFD GK 34 32a GGYS; P 53 48 [SJGYYG PEC ... Q 49b GTGYVGYTG R 55a GSSYN- -- C 1C K Others 73a [£SADK] FGDYTCDFRPGXFGPE(C/Q} . . 69b GGYGKFNDYLE. . form both complete and truncated motifs. Tyrosine typ- ically occurs in submotif #2, GGYSG, in the company of small residue
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
