. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 12 18 24 30 36 42 48 = 200 2 o Q. 150 100 50 B. 12 18 24 30 Time (h) 36 42 48 Figure 3. Incorporation of ['HJleucme in the presence or absence of emetine (9 pM) or anisomycin (200 n\I) and chioramphenicol (150 nM) as a function of time following addition of emetine. Larvae were pulsed at the times after addition of emetine or anisomycin indicated for 2 h ( jiCi/ml). In the chloramphemcol-treated samples, chlor- amphenicol was added 3 h prior to pulse-labeling. Mean values are dis- placed slightly on the abscissa for clari
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 12 18 24 30 36 42 48 = 200 2 o Q. 150 100 50 B. 12 18 24 30 Time (h) 36 42 48 Figure 3. Incorporation of ['HJleucme in the presence or absence of emetine (9 pM) or anisomycin (200 n\I) and chioramphenicol (150 nM) as a function of time following addition of emetine. Larvae were pulsed at the times after addition of emetine or anisomycin indicated for 2 h ( jiCi/ml). In the chloramphemcol-treated samples, chlor- amphenicol was added 3 h prior to pulse-labeling. Mean values are dis- placed slightly on the abscissa for clarity. (A) 4-day-old larvae were used (6 days old by the end of the experiment in the last 3 sets of samples). No antibiotic (diamonds); emetine (rectangles); emetine plus chioram- phenicol (triangles). (B) 5-day-old larvae were used (7 days old by the end of the experiment in the last 3 sets of samples). No antibiotic (dia- monds); anisomycin (rectangles); anisomycin plus chloramphenicol (tri- angles). addition of GABA, and plantigrade attachment followed. Attached larvae exhibited normal pedal locomotion in the presence of emetine. Abscission of the velum was also observed in the presence of emetine and occurred whether GABA (10~6 A/) was present or not, although at 9 emetine abscission occurred at lower levels when GABA was not present. Abscission was often premature or in- complete, particularly at higher concentrations of emetine. By 6 h after the addition of 10~6 M GABA, most of the larvae had settled both in the presence and absence of 9 juMemetine (Fig. 5A). New shell growth was not observed in the presence of emetine when larvae were induced to settle with 10~6 M GABA, although it was observed nor- mally in settled larvae in the absence of the inhibitor by 48 h. Attachment proceeded more rapidly at the higher. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these
Size: 2051px × 1218px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
