. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. 112 A. M. GLAUERT AND E. M. BRIEGER. Fig. 1. Ultra-tliin section of avian tubercle bacilli embedded in methacryiate. The organisms are distorted. Note the complex nuclear apparatus and large cytoplasmic granules. 110,000. If the original inoculum is viewed directly, without sectioning, it is seen to consist mainly of short rods with electron-dense polar bodies and a transparent, structureless cytoplasm. After 24 hours' growth these rods elongate and round bodies are clearly visible within them
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. 112 A. M. GLAUERT AND E. M. BRIEGER. Fig. 1. Ultra-tliin section of avian tubercle bacilli embedded in methacryiate. The organisms are distorted. Note the complex nuclear apparatus and large cytoplasmic granules. 110,000. If the original inoculum is viewed directly, without sectioning, it is seen to consist mainly of short rods with electron-dense polar bodies and a transparent, structureless cytoplasm. After 24 hours' growth these rods elongate and round bodies are clearly visible within them. These bodies have distinct membranes and it was suggested that they might correspond to the segmentation spores of the Nocardia. Methacryiate.—Sections of the small, transparent rods of the inoculum show that they have large central light areas containing threads and granules, the "nuclear apparatus". The cytoplasm, which occupies a narrow peripheral zone, has a granular structure, the granules ranging in size from 100 to 200 A. There is some evidence of the presence of fine threads and membranes within the cytoplasm but the cells are not sufficiently well preserved for these to be accepted with any certainty. The cells are obviously swollen and distorted and nothing definite can be deduced concerning the nature of the nuclear material. After 24 hours" incubation the rods of the inocu- lum have elongated, the dense threads and granules in the nuclear region have more complex configura- tions and the cytoplasm is denser (fig. I). The cyto- plasm still has a granular structure and in some organisms there is also a scattering of larger granules of 200 to 400 A diameter. A few of the organisms are in process of dividing by binary fission to form two daughter cells, each with its own nuclear appa- ratus. At later stages in the development of the bacilli a very different pattern of internal structure is seen. Rows of round bodies are observed forming inside the bac
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