. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B kDa i a. Figure 6. Immunostaining with antibodies affinity purified from SDS-PAGE bands that had OHSS activity. SDS-PAGE of the proteins from molecular-sieve fractions 6 and 7 (see Fig. 2A) was carried out for either h (A) or 9h (B), the longer electrophoresis producing the greater separation between the active bands at 32 kDa and 30 kDa. The bands were cut out of the PVDF membranes, incubated with a polyclonal antiserum raised to OHSS, and the bound antibodies were eluted (see Methods). These antib
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. B kDa i a. Figure 6. Immunostaining with antibodies affinity purified from SDS-PAGE bands that had OHSS activity. SDS-PAGE of the proteins from molecular-sieve fractions 6 and 7 (see Fig. 2A) was carried out for either h (A) or 9h (B), the longer electrophoresis producing the greater separation between the active bands at 32 kDa and 30 kDa. The bands were cut out of the PVDF membranes, incubated with a polyclonal antiserum raised to OHSS, and the bound antibodies were eluted (see Methods). These antibodies, obtained from the short and long electrophoresis, were applied to two new SDS-PAGE runs, both of h; these final runs are shown in this figure. Immunostaining was effected by antibodies bound (a) to protein in the upper band (32 kDa), and (b) to the protein in the lower band (30 kDa). The strips to the left of the two panels were stained with Coomassie brilliant blue (A) and Ponceau S (B); the numbers (with arrow) are the molecular masses of the standard molecules (identified in Fig. 2B).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
