. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 80 I. HORI *S»^' i£V".B & *il a%- x<-ii^. Figure 1. Low magnification \iew to demonstrate ruthenium red-positive areas in the subepidermal basal lamina. Three structural elements of the basal lamina are evident. Arrowheads indicate the cytoplasmic portion of a gland cell. Scale bar = 2 nm. Inset: High magnification view of the hemidesmosomal region. Ruthenium red staining. The limiting layer is stained clearly. Arrowheads indicate positive material sur- rounding microfibrils. Scale bar = ^m. Abbreviations: Ep
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 80 I. HORI *S»^' i£V".B & *il a%- x<-ii^. Figure 1. Low magnification \iew to demonstrate ruthenium red-positive areas in the subepidermal basal lamina. Three structural elements of the basal lamina are evident. Arrowheads indicate the cytoplasmic portion of a gland cell. Scale bar = 2 nm. Inset: High magnification view of the hemidesmosomal region. Ruthenium red staining. The limiting layer is stained clearly. Arrowheads indicate positive material sur- rounding microfibrils. Scale bar = ^m. Abbreviations: Ep, epidermal cell; P. cytoplasmic process; E, electronlucent zone; L. limiting layer: M. microfibrillar layer; Mf, muscle fiber; H. hemidesmosome. istry. Denv its. indicating binding of the antibody to laminin. i served on the basal lamina. The de- posits were esp prominent on its epidermal side (Fig. 2). Simila, s were also seen in the same region of the 6-day regeno 4). The control sections in- cubated with TBS should no reactive deposits in the basal lamina (Fig. 3). Immunoelectron microscopic observations To resolve the localization of laminin in the basal lam- ina, immunoelectron microscopy was applied to Lowicryl- embedded thin sections. The ABC method showed dense immunoreactivity on the limiting layer and on a part of the electronlucent zone along the limiting layer (Fig. 5).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
