Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . reptothrix ep-pingeri, in the sputum of cases showing the clinical symptoms of pulmonarytuberculosis. These organisms are about four times as thick as the tuberclebacillus; when stained they are resistant to decolorization by acids, but areslowly decolorized by strong alcohol. Stained specimens are easily made with 1 Boston (Interstate Med. Journ., 1914, XXI, 330) reports the relatively frequent presenceof tubercle bacilli in the sputum of acute colds, with disappearance of these organ
Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . reptothrix ep-pingeri, in the sputum of cases showing the clinical symptoms of pulmonarytuberculosis. These organisms are about four times as thick as the tuberclebacillus; when stained they are resistant to decolorization by acids, but areslowly decolorized by strong alcohol. Stained specimens are easily made with 1 Boston (Interstate Med. Journ., 1914, XXI, 330) reports the relatively frequent presenceof tubercle bacilli in the sputum of acute colds, with disappearance of these organisms duringconvalescence. * See Claypole, Jour. Exper. Med., 1912, XVII, 99. ^ See Kato, Mitt. a. d. med. Fakultiit der k. Univ., Tokyo, 1915, XIII, 441. THE SPUTUM. 15 the use of methylene-blue dyes or of Grams method. The leptothrix groupis particularly abundant in the mouth and is found in large numbers in the lungsin cases of pulmonary gangrene. Yeast fungi occur in the sputum at times, but rarely in the fresh specimens. ^They are oval or elliptical cells and are very refractive, sometimes resembling. Fig. 3.—Aspergillus fumigatus. very closely fat droplets. Their appearance may vary from that of a simpleoval cell without distinct limiting membrane to those with definite membraneand vacuoles. These cells are especially characterized by their tendency tothrow out projections or buds at various points of their periphery. They varyin size from i to 40 microns in diameter. While these yeast fungi are usually
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