. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 700 ~ O 6 -zi DA C -22- C Molt stages L stages 20 25 Days Figure3. Salinity tolerance in zoeae [-3 of Cancer borealis at 15°C. Variations in LS 50 in %» and mosm • kg"' according to larval and molt stages and to days of development, in high and low salinities (upper and lower traces). Each point represents the mean value of two determinations from 10 animals, with 95% confidence interval. Closed triangles: 24 h LS 50; closed circles: 48 h LS 50; open circles: 96 h LS 50. seawater, and their regulation was slightly hyper
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 700 ~ O 6 -zi DA C -22- C Molt stages L stages 20 25 Days Figure3. Salinity tolerance in zoeae [-3 of Cancer borealis at 15°C. Variations in LS 50 in %» and mosm • kg"' according to larval and molt stages and to days of development, in high and low salinities (upper and lower traces). Each point represents the mean value of two determinations from 10 animals, with 95% confidence interval. Closed triangles: 24 h LS 50; closed circles: 48 h LS 50; open circles: 96 h LS 50. seawater, and their regulation was slightly hyper-osmotic in dilute media (Fig. 5). No difference of hyper-regulation was detected between large and small adults in C. bomilis. In C. irroratus large crabs were significantly stronger reg- ulators than small crabs (hemolymph-medium differences of 71 ± 7 and 55 ± 13 mosm • kg"1 respectively, P < , in a 500 mosm • kg"1; 17%o, medium). The ability to hy- per-regulate in dilute media was significantly higher in C. irroratus than in C. borealis (in large crabs, hemolymph- medium differences of 71 ±7 and 37 ± 5 mosm-kg ', P < , in a 500 mosm-kg"1, 17%o, medium). Zoeae 1 to 5 of C. irroratus in molting stage C hyper- osmoconformed at almost all tested salinities, , their hemolymph osmotic pressure varied as a function of ex- ternal osmotic pressure but remained above external by about 15-50 mosm • kg"1; at the lowest tested salinity (300 mosm • kg"1), zoeae were isosmotic. In some zoeal stages. regulation in dilute media was slightly more hyper-osmotic in premolt (zoeae 1,4 at 500 mosm • kg"': P < ) and less hyper-osmotic in post-molt (zoeae 2, 3, 4 at 500 and 900 mosm • kg"': P < ). Megalopae were also hyper- osmoconformers. The pattern of osmoregulation seemed to change after the completion of metamorphosis. First crab stages were almost osmoconformers in seawater and their regulation was slight
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology