. Biological structure and function; proceedings. Biochemistry; Cytology. THE FUNXTIONAL LINK OF SUCCINIC DEHYDROGENASE 195. [Oj]=0 Fig. 2. Stimulation of succinate oxidation by ATP in rat liver mitochondria preincubated with arsenate and dicoumarol [5]. Experimental conditions as in Fig. I. 0001 M ATP (ATP) was added at the point indicated. oxidation, as a consequence of the resynthesis of high energy intermediates taking place during the oxidation of succinate. When this resynthesis was aboUshed by the presence of an uncoupling agent, the depression of succinate oxidation became permanent. U
. Biological structure and function; proceedings. Biochemistry; Cytology. THE FUNXTIONAL LINK OF SUCCINIC DEHYDROGENASE 195. [Oj]=0 Fig. 2. Stimulation of succinate oxidation by ATP in rat liver mitochondria preincubated with arsenate and dicoumarol [5]. Experimental conditions as in Fig. I. 0001 M ATP (ATP) was added at the point indicated. oxidation, as a consequence of the resynthesis of high energy intermediates taking place during the oxidation of succinate. When this resynthesis was aboUshed by the presence of an uncoupling agent, the depression of succinate oxidation became permanent. Under these conditions, added ATP was required for stimulating the oxidation of succinate (Fig. 2). No stimulation of the oxidation rate was observed when ATP was replaced by AMP or EDTA. TABLE I Depletion of Mitochondrial Endogenous Phosphate by Arsenate and Protection by Succinate "*-P-labelled mitochondria" from 500 mg. rat liver (wet weight) were incubated in open tubes at 30. After 7 min., i ml. of the incubation mixture was filtered through a Celite layer as reported elsewhere [12]. Each tube contained in a final volume of 5 ml.: 0-05 M KCl, 0-03 M tris buffer pH 7-5, 0-125 ^ sucrose, and when indicated o-oi M succinate, 0-003 ^' arsenate, 2 /(g. antimycin A, o-ooi M KCN. As in Table II and III, the number of counts is here indicative of the amounts of endogenous phosphate which remained in the mitochondria after preincubation. Additions Counts/min. ( x 10 ^) None Arsenate Succinate Arsenate + succinate Arsenate + succinate + Antimycin A Arsenate + succinate + KCN 700 100 (14•4°,,) 521 (73-5"o) 421 (6o-2<'o) 223 (3i-9"o) 128 (18-30,.). Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry;
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