. Pathological technique; a practical manual for workers in pathological histology and bacteriology. 3. Wash with Grams I. K. I. solution. 4. Wash with formalin (40 per cent, formaldehyde solu-tion). 5. Decolorize with 95 per cent, alcohol. 6. Wash quickly with Grams I. K. I. solution. 7. Cover with a special eosin mixture (see below), warm-ing in the flame for a few seconds. 8. Wash, dehydrate with alcohol, clear with xylol, andmount in balsam. To obtain the best results the duration of the applicationof the various reagents must be varied with each prepara-tion, and in some instances, where
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. 3. Wash with Grams I. K. I. solution. 4. Wash with formalin (40 per cent, formaldehyde solu-tion). 5. Decolorize with 95 per cent, alcohol. 6. Wash quickly with Grams I. K. I. solution. 7. Cover with a special eosin mixture (see below), warm-ing in the flame for a few seconds. 8. Wash, dehydrate with alcohol, clear with xylol, andmount in balsam. To obtain the best results the duration of the applicationof the various reagents must be varied with each prepara-tion, and in some instances, where very deep staining isdesired, the stronger solution of I. K. I., Lugols solution,may give better results. 252 PATHOLOGICAL TECHNIQUE. The decolorization by alcohol may have to be supple-mented by washing with ether or with aniline-xylol, for theGram staining may be so intense as to mask the red stain-ing capsules. This is particularly true in the case of Strep-tococcus viridans or streptococci in certain cases of endo-carditis in which a very narrow capsule may be demon-strable by this Fig. 30.—Streptococci with capsules in a section of lung; X 2000 (W. H. Smithand L. S. Brown). The special eosin mixture is made by shaking 1 part ofaniline green in 200 parts of a 3 to 6 per cent, aqueoussolution of yellowish water-soluble eosin and, after one ortwo hours standing, filtering to remove the precipitate. PATHOGENIC BACTERIA AND FUNGI. The number of species of bacteria of pathogenic signif-icance which are commonly encountered in pathological pro-cesses in man is a small one. These comprise the staphylo-coccus pyogenes aureus, the streptococcus pyogenes, thepneumococcus, the bacillus coli communis, the typhoid ba-cillus, the bacillus diphtheriae, and the bacillus is with infections with these few species that the patholo-gist is most frequently concerned, and the determination ofthe presence of these alone comprises by far the greater partof the bacteriolo
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