. Cultural studies of species of Penicillium. Penicillium -- Cultures and culture media; Fungi -- Cultures and culture media. 48 CULTURAL STUDIES OF SPECIES OF PENICILLIUM. CULTURAL DATA. Same as P. brevicaule Sacc, except the following difference noted: Conidia cream, somewhat larger than P. brevicaule. Cohn's solution failed to produce characteristic colonies. In Dox's solution, with butterfat as a source of carbon, it differs from P. brevicaule by- failing to cause drops of yellow oil to separate out. Agar-agar: In repeated cultures this organism has failed to grow well in agar media. In so
. Cultural studies of species of Penicillium. Penicillium -- Cultures and culture media; Fungi -- Cultures and culture media. 48 CULTURAL STUDIES OF SPECIES OF PENICILLIUM. CULTURAL DATA. Same as P. brevicaule Sacc, except the following difference noted: Conidia cream, somewhat larger than P. brevicaule. Cohn's solution failed to produce characteristic colonies. In Dox's solution, with butterfat as a source of carbon, it differs from P. brevicaule by- failing to cause drops of yellow oil to separate out. Agar-agar: In repeated cultures this organism has failed to grow well in agar media. In some such cases the spores transferred from the agar to gelatin grew at once. Some cultures upon agar grow slowly and in typical manner, but the development upon all agar media has seemed uncertain. In synthetic solution cultures were obtained when the inoculation of tubes of the same solution with per cent of agar added to make a solid substratum produced no growth. PENICILLIUM BREVICAULE Saccardo, var. GLABRUM Thorn, n. var. Colonies white or only slightly yellowish-tinged in all gelatin media, grow not at all or with difficulty on agar of most formulae. Aerial portion consisting of short, closely crowded conidiophores making a powdery surface overgrown by loosely trailing hyphte and ropes of hyphse, spreading broadly over the substratum. Conidiophores, short, mostly 10-30^, arising directly from submerged hyphse or numerously and irregu-. Fig. 14.—Penicillium brevicaule, var. glabrum: a, b, c, branching of conidial fructification (X 900); d, chain of conidia (X 1,400); /, formation of conidium on young branch (X 1,400); g, h, sketch of appearance in culture (X 140); m, n, o, germination of conidia. larly borne as perpendicular branches of the superficial hyphse and ropes of hyphse. Conidial fructifications from simple chains of spores to fairly complex penicillate groups of branchlets resembling P. brevicaule, but mostly less complex. Conidia obovate, pyriform 7-8 by 8-1
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