. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 6. AFLP assay of zooxanthella samples. The ATG primer was used after PCR preamplification of zooxanthella templates (see methods). Identical conditions for AFLP analyses were used on both Zooxanthellae and coral (Montaxtraea faveolala) DNA samples. Three faint bands (indi- cated by arrows) obtained from Type A Zooxanthellae appear similar to the three dominant AFLP bands obtained from M. faveolata (550, 630. 750 bp) shown in Fig. IB; these may be due to coral (animal) contamination of the zooxanthella DNA. Allele size
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 6. AFLP assay of zooxanthella samples. The ATG primer was used after PCR preamplification of zooxanthella templates (see methods). Identical conditions for AFLP analyses were used on both Zooxanthellae and coral (Montaxtraea faveolala) DNA samples. Three faint bands (indi- cated by arrows) obtained from Type A Zooxanthellae appear similar to the three dominant AFLP bands obtained from M. faveolata (550, 630. 750 bp) shown in Fig. IB; these may be due to coral (animal) contamination of the zooxanthella DNA. Allele size*/pattern Species 160 \W 160/169 Null Total M. franksi X 1 1 1 11 M. annularis 7 2 1 1 II M. faveolata 0 1 0 11 12 * Sizes indicated are approximate (see legend Fig. 7). Discussion Status of the three members of the Montastraea annularis complex When the specific status of taxa in sympatry is question- able, multiple, independent, fixed differences provide com- pelling evidence for the lack of effective interbreeding (Avise and Ball, 1990). The reciprocal presence/absence pattern for the GGAG 880 and 920 bp bands appears to represent one such fixed difference between M. faveolata and the other two taxa. In addition, strong frequency differ- ences at the ATG 630 locus and failure to amplify the 160 or 169 bp alleles at the microsatellite locus in most M. faveolata also point to the distinctiveness of this species. The significance of these genetic differences is further sup- ported by other biological differences that distinguish the taxa (Tomascik, 1990: Hayes, 1990; Knowlton et ai, 1992; Van Veghel and Bak, 1993, 1994; Van Veghel. 1994; Van Veghel and Kahmunn, 1994; Van Veghel and Bosscher, 1995; Van Veghel et ai. 1996; Weil and Knowlton, 1994; Szmant et ai, 1997; Knowlton et 1997; Knowlton and Budd, unpubl.). M. faveolata M. franksi M. annularis. bp 200 154 Figure 7. A subset of the Monlastraea samples assayed for the Mfra-gtttl microsatellite locus. Sample number and sp
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
