. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. POPULATION GENETICS OF A MEIOFALINAL POLYCHAETE 219. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Figure 2. RAPD fingerprinting of Hesionides gohari specimens from Phuket (2. 31, Florida (8-11), Crete (12-151. Giglio(16-18). Arcachon (19-23). and H. riegerorum specimens from North Carolina (4-7); 1 and 24; 100 bp marker. Primer OPB 6. Technologies, Inc., Berlin, Germany, or Pharmacia Biotech, Uppsala, Sweden). The reproducibility of the data was checked at regular intervals on different levels throughout al
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. POPULATION GENETICS OF A MEIOFALINAL POLYCHAETE 219. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Figure 2. RAPD fingerprinting of Hesionides gohari specimens from Phuket (2. 31, Florida (8-11), Crete (12-151. Giglio(16-18). Arcachon (19-23). and H. riegerorum specimens from North Carolina (4-7); 1 and 24; 100 bp marker. Primer OPB 6. Technologies, Inc., Berlin, Germany, or Pharmacia Biotech, Uppsala, Sweden). The reproducibility of the data was checked at regular intervals on different levels throughout all three parallel experiment lines. First, at the beginning of each series the optimal DNA concentration for PCR was determined for the individual animal, by testing the PCR with three DNA concentrations per animal (cci. 1 ng, 3 ng, and 10 ng per 25 jul reaction volume). In pilot experiments with larger annelids, it became clear that the results were reproducible with up to 25 ng/25 /u,l reaction volume. With DNA concentrations as high as ca. 50 ng/25 reaction volume, reproducibility was distinctly worse. A further in- crease in DNA concentration (to over 100 ng/25 reaction volume) brought the PCR to a complete halt, so that no specific DNA fragments could be detected. Reproducibility was tested further throughout each test series by running the same experiment twice in parallel reactions. In addition, for each series one reaction was carried out with a "blind" sample lacking DNA, to check the possibility of contami- nation with foreign DNA. We also looked for differences in the amplification patterns that might derive from the use of different thermocyclers (Biischer et al., 1993; He et 1994) or preservation methods (drying or deep-freezing), but found none. The detected amplification product patterns were exam- ined visually for monomorphic and polymorphic markers (Hadrys et 1992). The degrees of polymorphism are given in percentages. The banding
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
