. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. .£ 350 aposymbiotic symbiotic Figure 7. Specific activities (U/mg soluble protein) ofCu-Zn SOD and Mn SOD in symbiotic and aposymbiotic Aifiiasia pulchella. Six anemones were homogenized individually. From each homogenate, samples were taken for SOD measurement. Each bar represents the mean ± SD of the six homogenates. mal stress, net oxygen production by intact anemones ceased (32°C; Table I). After 60 min, intact anemones began to consume oxygen in the light at rates compara- ble to their dark respiration rates at elevated
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. .£ 350 aposymbiotic symbiotic Figure 7. Specific activities (U/mg soluble protein) ofCu-Zn SOD and Mn SOD in symbiotic and aposymbiotic Aifiiasia pulchella. Six anemones were homogenized individually. From each homogenate, samples were taken for SOD measurement. Each bar represents the mean ± SD of the six homogenates. mal stress, net oxygen production by intact anemones ceased (32°C; Table I). After 60 min, intact anemones began to consume oxygen in the light at rates compara- ble to their dark respiration rates at elevated temperature (Table I). Net oxygen flux in FIZ also decreased dramatically within 20 min after exposure to elevated temperature (Table I). This decrease was accompanied by an increase in dark respiration. There was considerable variability in the mean response of FIZ to elevated temperature. In c 6000 - 1 a- 5000 - u 12 •f 4000 - tn T 00 - 3000 - •f ' 2000 ' ^ 1000 - 1 r=_1 GO aposymbiotic symbiotic Figure 8. Specific activity (U/mg soluble protein) of CAT in sym- biotic and aposymbiotic Aipla\ia pulchella. Six anemones were homog- enized individually. From each homogenate. samples were taken for CAT measurement. Each bar represents the mean ± SD of the six ho- mogenates. o S. 300- -I 250 ao <N O 200- 150- 100- 50- 0 32°C. 0 20 40 time (mm) 60 Figure 9. Time course of the dark respiration response of intact anemones to acute thermal stress. Dark respiration by an individual anemone was measured first at 26°C over 60 min (at and 60 min). The 26°C seawater was then immediately replaced with seawater pre- heated to 32°C. Dark respiration was then measured for another 60 min at 32°C (at 1, 20. and 60 min). Each point represents the mean ± SEM for three anemones. three of the five experiments performed, net oxygen con- sumption in the light began within 20 min of exposing the FIZ to elevated temperature. Although net oxygen production was observed in the two r
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
