. Cottony rot of lemons in California. ium was placed without crushingit in 10 cc. of sterilized water contained in a test tube. After 15 min-utes the water was well infected with cottony rot spores, and theapothecium was removed. From this spore-laden liquid, one loopful(2 mm. diameter loop) was placed in each of the test tubes containing10 cc. of copper sulphate to be tested. After the required length oftime that the copper sulphate solution was to act on the spores, theentire contents of the tube was poured into a flask of bread and prunejuice medium. The following shows the strengths in pe
. Cottony rot of lemons in California. ium was placed without crushingit in 10 cc. of sterilized water contained in a test tube. After 15 min-utes the water was well infected with cottony rot spores, and theapothecium was removed. From this spore-laden liquid, one loopful(2 mm. diameter loop) was placed in each of the test tubes containing10 cc. of copper sulphate to be tested. After the required length oftime that the copper sulphate solution was to act on the spores, theentire contents of the tube was poured into a flask of bread and prunejuice medium. The following shows the strengths in per cent usedand the time that the spores were acted upon by the germicide: 254 UNIVERSITY OF CALIFORNIA EXPERIMENT STATION Per cent 3 min. 5 min. 10 min. 15 min. 1/5 5 tubes 5 tubes 5 tubes 5 tubes 1/10 5 tubes 5 tubes 5 tubes 5 tubes 1/25 5 tubes 5 tubes 5 tubes 5 tubes 1/50 5 tubes 5 tubes 5 tubes 5 tubes April 11th, 1912. All the different strengths tested in bread-primejuice medium showed characteristic growth of cottony rot. Hence.
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