. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 122 A. E. CARPIZO-ITUARTE AND HADFIELD 80x10' 103 - D, 40x103 2 a. 101 - 0 250 500 1000 Concentration of emetine (nM) B. SOxlO 5. 60x1O3 - E 40x10' n. -a 20x10-' - no emetine emetine 1 uMolar O O 1 4 6 Period of incubation (h) Figure 111. Incorporation of 33S-methionine into newly synthesized proteins by larvae of HyJmitles elegans in the presence of the translation inhibitor emetine. In (A), larvae were incubated in varying concentrations of emetine, shown on the _v axis, for 4 h with incorporation of "S
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 122 A. E. CARPIZO-ITUARTE AND HADFIELD 80x10' 103 - D, 40x103 2 a. 101 - 0 250 500 1000 Concentration of emetine (nM) B. SOxlO 5. 60x1O3 - E 40x10' n. -a 20x10-' - no emetine emetine 1 uMolar O O 1 4 6 Period of incubation (h) Figure 111. Incorporation of 33S-methionine into newly synthesized proteins by larvae of HyJmitles elegans in the presence of the translation inhibitor emetine. In (A), larvae were incubated in varying concentrations of emetine, shown on the _v axis, for 4 h with incorporation of "S- methionine during the last h. Points are means of methionine incorpo- ration ± 1 SE (H = 2 replicates/treatment). In (B). larvae were incubated in 1 emetine for the intervals indicated on the .v axis and allowed to incorporate "S-methionine for an additional 45 min. Points are mean methionine incorporation ± 1 SE. morphose with either a bacterial biotilm or a pulse of CsCl in the presence of the translation inhibitor emetine, just as with DRB, they completed metamorphosis and stopped developing before the outgrowth of the branchial radioles. Neither the highest concentration of emetine used (1 juM) nor an extended preincubation period in emetine stopped initiation of mctamorphic morphogenesis in response to a bacterial biofilm or cesium as inducers. These results are consistent with the hypothesis that initiation of metamor- phosis in H. elegim* is also highly independent of synthesis of new proteins, a result that is concordant with previous studies of other species, especially certain molluscs (Fen- teany and Morse, 1993; Del Carmen and Hadtield. 1999). Larvae of the archaeogastropod Haliotis ntfescens (Fenteany and Morse, 1993) and the nudibranch Pliestilla sibogae (B. J. McCauley, 1997; Del Carmen and Hadfield, 1999) initiate metamorphosis in the presence of translation inhibitors. When competent larvae of H. nifescens were induced to metamorphose in the p
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
