. Biological structure and function; proceedings. Biochemistry; Cytology. NUCLEOTIDES AND MITOCHONDRIAL FUNCTION 235 oxidizing effect of ATP, which otherwise is very small. Azide at this concentration does not yet inhibit noticeably the respiration (cf. also [i6]). The reductive effect of ATP on cytochrome h and the oxidative effect of ATP on cytochromes c and a are reversed by addition of phosphate, ADP or dinitrophenol, as was found with the ATP-dependent reduction of DPX and Fig. 7. The simultaneous inhibition of respiration and oxidation of cyto- chrome a in the presence of
. Biological structure and function; proceedings. Biochemistry; Cytology. NUCLEOTIDES AND MITOCHONDRIAL FUNCTION 235 oxidizing effect of ATP, which otherwise is very small. Azide at this concentration does not yet inhibit noticeably the respiration (cf. also [i6]). The reductive effect of ATP on cytochrome h and the oxidative effect of ATP on cytochromes c and a are reversed by addition of phosphate, ADP or dinitrophenol, as was found with the ATP-dependent reduction of DPX and Fig. 7. The simultaneous inhibition of respiration and oxidation of cyto- chrome a in the presence of succinate on addition of ATP. Amperometric record- ing of oxygen consumption and simultaneous spectrophotometric recording at the a-band of cytochrome a. The interaction of ATP with the respiratory chain causes a "crossover point" of the redox changes of the respiratory components between cyto- chrome h and cytochrome c. The "crossover point" can be interpreted to indicate the reaction step at which the electron transfer is inhibited by ATP. Thus, it is to be expected that in the overall electron transport also, in the oxygen uptake, an inhibitory effect of ATP can be observed. As shown in the upper recordings of Figs. 7 and 8, after addition of ATP, the respiration of skeletal-muscle mitochondria, both with glvcerolphosphate and succinate, is inhibited to about 50^'o- After further addition of phos- phate or dinitrophenol, respiration is again accelerated synchronously with. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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