. The principles of bacteriology: a practical manual for students and physicians. and more dense. A^ hen of about theconsistency of fresh solid rubber, or preferably not quiteso dense, it is ready to cut. A small portion, about halfa cubic centimetre, should be cemented to a bit of corkwith ordinary mucilage, and allowed to remain in theopen air for a minute or two for the mucilage to should be dropped upon it occasionally to pre-vent drying of the tissue. When the mucilage is firm 1 The details of the method of decolorizatjou will be described iu thegectiOD on the technique of
. The principles of bacteriology: a practical manual for students and physicians. and more dense. A^ hen of about theconsistency of fresh solid rubber, or preferably not quiteso dense, it is ready to cut. A small portion, about halfa cubic centimetre, should be cemented to a bit of corkwith ordinary mucilage, and allowed to remain in theopen air for a minute or two for the mucilage to should be dropped upon it occasionally to pre-vent drying of the tissue. When the mucilage is firm 1 The details of the method of decolorizatjou will be described iu thegectiOD on the technique of staining, 184 BACIEBIOLOGY. the cork with the piece of tissue upon it may be left inalcohol over night, and on the following day the sec-tions may be cut. Section-cutting.—This is accomplished by the useof an instrument known as a microtome. In Fig. 36is seen the form now commonly employed. It is knownas Schanzes microtome. It is an apparatus provided witha clamp for holding the cork upon which the tissue is ce-mented, and also a sliding clamp which carries a knife. Fig. Schanzes microtome. The tissue is clamped horizontally, and the knife is causedto slide across its upper surface, also in a horizontal the clamp for holding the tissue is a milled disk,by means of which a screw is caused to revolve, and inrevolving raises or lowers the clamp holding the tissue,so that the tissue may be brought closer to or fartherfrom the plane in which the knife slides. By thisarrangement sections of any desired thickness can be SECTION-CUTTINO. 185 cut by turning the milled disk with the one hand andcausing the knife to traverse the tissue with the tissue and the knife-blade should be kept wetwith alcohol, so that the sections may float upon theblade of the knife, from ^vhich they can easily be re-moved, without tearing, A\ith a curved needle or axiamels-hair brush. As the sections are cut they areplaced in a dish containing alcohol. There are some tissues which
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbacteri, bookyear1902
