. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LUMINESCENCE CONTROL IN CHAETOPTERVS 585 into the electrode tip which was polished over an alcohol burner. To avoid interference arising from the large quantities of mucus produced by the worm, good electrode contact was achieved by connecting the latter to a suction chamber; suction was controlled by connecting the chamber to a three-way valve and a 1-ml disposable syringe. The suction chamber was placed on a micromanipulator for precise positioning of the electrode tip viewed through a dissecting microscope. Signals were f
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LUMINESCENCE CONTROL IN CHAETOPTERVS 585 into the electrode tip which was polished over an alcohol burner. To avoid interference arising from the large quantities of mucus produced by the worm, good electrode contact was achieved by connecting the latter to a suction chamber; suction was controlled by connecting the chamber to a three-way valve and a 1-ml disposable syringe. The suction chamber was placed on a micromanipulator for precise positioning of the electrode tip viewed through a dissecting microscope. Signals were fed to a Grass P511 preamplifier whose bandwidth was adjusted between 1 and 30 Hz. The amplified signals were received by a Tektronix 5113 oscilloscope for instantaneous visualization and by a Grass 79D polygraph for per- manent records. Stimulation Electrical stimulation was applied through fine teflon-coated platinum electrodes which were connected to a Grass stimulation isolation unit and fed with square pulses from a Grass S44 stimulator. Mechanical stimulation of limited epithelial areas of the parapods was delivered through a glass rod with a smooth, rounded end. The rod was clamped to a galva- nometer (Grass 70SC D'Arsenval oscillograph). The application of current (10- 50V) from a Grass stimulator to the galvanometer allowed control over the amplitude and duration of the displacement of the probe. The force delivered by this experimental set-up ranged between (10V) and dyne (50V) as estimated with a Grass force transducer. RESULTS Electrical stimulation Suprathreshold stimulation of any serial parapod by a single pulse led to a local luminescent response. As stimulus intensity was increased, luminescence spread to neighboring parapods, both anteriorly and posteriorly. First, ipsilateral parapods were recruited followed by contralateral parapods at higher stimulus intensities (Fig. 1).. Please note that these images are extracted from scanned page i
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
