. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. — >- - — -O— - — -H— - ---- H ---- ft pulclira (polar 10 nl/ml ) D fitichra (polar 1 (il/ml) D pulclirti (plant) O. pulclira [nonpolar 1 iig/mll D pulchra (nonpolar 100 ng/ml) £ rtii/id/a (polar 10 nl/ml) E raduita (plant) £. radwia (nonpolar 1 [ig/ml) DMSO control () MilhQ control Seawater control Figure 2. Percent (A) settlement and (B) metamorphosis over time of competent Holopneusles purpunis- ccns (one larva per replicate; 10 replicates per treatment) when exposed to pieces of Delixi'ii pulclirti (15 mg per rep
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. — >- - — -O— - — -H— - ---- H ---- ft pulclira (polar 10 nl/ml ) D fitichra (polar 1 (il/ml) D pulclirti (plant) O. pulclira [nonpolar 1 iig/mll D pulchra (nonpolar 100 ng/ml) £ rtii/id/a (polar 10 nl/ml) E raduita (plant) £. radwia (nonpolar 1 [ig/ml) DMSO control () MilhQ control Seawater control Figure 2. Percent (A) settlement and (B) metamorphosis over time of competent Holopneusles purpunis- ccns (one larva per replicate; 10 replicates per treatment) when exposed to pieces of Delixi'ii pulclirti (15 mg per replicate) and its polar and nonpolar extract fractions. Controls ot'MilliQ water and sterile, filtered seawater were used. rculititti, no settlement occurred in response to its polar or nonpolar extracts. At the end of the assay, no significant difference in settlement was observed between larvae ex- posed to treatments containing E. radiata. and the MilliQ and seawater controls (X: = , 5 df, P = ). Larval H. purpurascens metamorphosed in response to extracts from D. pulchra but not those from E. radiata (Fig. 2B). After 20 h, all larvae of H. purpurascens had meta- morphosed in the presence of pieces of D. pulchra and its polar extract, regardless of concentration (Fig. 2B). The polar extracts at both concentrations induced metamorpho- sis at a faster rate than did pieces of the alga. All larvae exposed to polar extracts from D. pulchra at 10 /xl ml"1, and the majority of larvae exposed to polar extracts from D. pulchra at 1 ml"1, metamorphosed within 3 h (Fig. 2B). Nonpolar extracts from D. pulchra also induced a low percentage of metamorphosis; however, this rate was sig- nificantly lower than that for both polar extracts (20 h: X2 = 3 df, P galactopyranosyl-( l-2)-glycerol] (Fig. 3). We were unable to further separate [ 1 ] using standard chromatographic techniques. High field two-dimensional NMR studies (COSYDQF, HMBC and NOESY) of
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
