Fungous diseases of plants . ifications in the host. A discussion of special biological or physiological relations hasbeen limited to a few topics. The germination of spores is fromthe outset one of the investigational or routine duties of the pathol-ogist ; the relations of the fungi to chief environmental factorscannot be disregarded; artificial infection is required in determiningthe causal organism ; and the principles of disease control are con-cerned with the immediate application of pathological study toeconomic purposes. PART I CULTURE METHODS AND TECHNIQUE CHAPTER I ISOLATION AND PURE
Fungous diseases of plants . ifications in the host. A discussion of special biological or physiological relations hasbeen limited to a few topics. The germination of spores is fromthe outset one of the investigational or routine duties of the pathol-ogist ; the relations of the fungi to chief environmental factorscannot be disregarded; artificial infection is required in determiningthe causal organism ; and the principles of disease control are con-cerned with the immediate application of pathological study toeconomic purposes. PART I CULTURE METHODS AND TECHNIQUE CHAPTER I ISOLATION AND PURE-CULTURE METHODS LOEFFLER, Fr. Voilesungeii iiber die geschichtliche Entwickelung der Lehrevon den Bakterien 1 : 252 pp. 3 pis. 37 figs. 1887. Leipzig. Smith, Erw. F. Bacteria in Relation to Plant Diseases. Carnegie Inst, ofWashington, Publication 27 (Vol. I): 285 pp. 31 ph. i^jfigs. 1905. (Text-Books and Manuals of Bacteriology.) Nearly all texts on general bac-teriology devote considerable space to methods of culture Fig. I. View in Lakoratory equipped for Plant Physiology andPathology. (Photograph by O. Butler) The student who is interested in the fungous diseases of plantswill find it desirable at the outset to acquire a knowledge of pure-culture methods. The investigator in plant pathology can only pro-ceed confidently in his work when he has had practical training in 9 lO CULTURE METHODS AND TECHNIQUE the cultivation of fungi in the laboratory. This work has becomeincreasingly more important in recent years. Laboratory culturemethods were not generally applied to a study of the filamentousfungi until some years after bacteriology had been revolutionizedby a series of important discoveries in this line of technique. It isat once evident that the bacteria could never be studied advanta-geously except through the establishment of pure-culture methods,whereas the larger fungi were to the early systematists and mor-phologists, organisms to be studied after the method ap
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