. The elements of pathological histology with special reference to practical methods . erilised glass plates, and spread outevenly thereon by means of the sterilised edge of the test-tube, insuch a manner, however, that the marginal portions of the platesremain free for a breadth of about 2 cm. In order that the gelatin may not flow off in one direction, it ismerely necessary that the plate should lie perfectly horizontal, which ismanaged by the use of what is known as the plate-pouring (Fig. 8) consists of a wooden triangle resting on levelling-screws,and having laid upon it a
. The elements of pathological histology with special reference to practical methods . erilised glass plates, and spread outevenly thereon by means of the sterilised edge of the test-tube, insuch a manner, however, that the marginal portions of the platesremain free for a breadth of about 2 cm. In order that the gelatin may not flow off in one direction, it ismerely necessary that the plate should lie perfectly horizontal, which ismanaged by the use of what is known as the plate-pouring (Fig. 8) consists of a wooden triangle resting on levelling-screws,and having laid upon it a larger plate of glass, which can be renderedperfectly horizontal by means of the screws controlled by a this large, plate the sterilised culture-plates (usually 12 and 6 cm. wide) are first deposited, being again covered with 40 BACTERIOLOGICAL METHODS a bell-glass. Furtherniore, the gelatin when poured out must not betoo fluid, and, lastly, its solidification can be accelerated to the greatestpossible extent by filling the wooden triangle with ice or cold Fl(i. 8.—AlPARATlS FUK MAKINIJ PlATE CULTIVATIONS. As soon as the gelatin has fully solidified, the plates are stored atroom temperature, that is, about 20 C, in a moist chamber, in whichindeed several can be placed one above the other upon glass benches(Fig. 7). In pouring out the gelatin on plates the germs of bacteriacontained in the former are widely separated from one another, acondition which, in consequence of being fixed by the solidifyinggelatin, they also retain during their development into , after several days, as many isolated colonies are, as arule, found upon the plates as there were germs originally present;and, inasmuch as each of these colonies consists of individuals of butone species, a pure culture of the original germ is obtained. If there was only a single species of bacterium in the original material,but one kind of colony will occur on the plates ; but
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectpatholo, bookyear1895
