. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Phenyl Methane Dyes 155 temperature (85°C.), fuming sulfuric acid containing sufficient free SO3 to insure complete sulfonation to the trisulfonic acid, and careful acidification to yield only the disodium salt. Since the method which they proposed in this article was called to the atten- tion of manufacturers, the American product has improved; it not only gives permanent Van Gieson preparations, but proves more satisfactory in
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Phenyl Methane Dyes 155 temperature (85°C.), fuming sulfuric acid containing sufficient free SO3 to insure complete sulfonation to the trisulfonic acid, and careful acidification to yield only the disodium salt. Since the method which they proposed in this article was called to the atten- tion of manufacturers, the American product has improved; it not only gives permanent Van Gieson preparations, but proves more satisfactory in the Mallory connective tissue stain. The spectrophotometric curve of a typical acid magenta I (rosanilin) is given in Fig. 20 (curve 2), p. 149. The chief dif- ference between this and the corresponding basic fuchsin is that it has a distinct secondary maximum at about 500, where the curve for basic rosanilin shows merely an almost imperceptible bulge. A dye having its primary maximum at 500 would be orange in shade, which is approximately the color of the acid phase of the Andrade indicator. PROCEDURES RECOMMENDED BY THE COMMISSION IN WHICH THIS STAIN IS USED PAGE REFERENCE TO NAME OF PROCEDURE Staining Procedures Van Gieson stain with iron hematoxylin IA4-21 Mallory's anilin blue collagen stain IB3-3 Grossman's modification IB3-5 Acid alizarin blue modification IB3-6 Masson's trichrome stain IB3-I8 Pianese IIIB stain for fungi in plant tissue IIA-12 n20 HOFMANN S violet C. I. NO. 679 Synonyms: Dahlia. Iodine violet. Red violet. Violet R, RR or 4RN. Primula R water soluble. These various names are applied in a somewhat indiscriminate way to dyes intermediate in shade between basic fuchsin and methyl violet. Theoretically they are mixtures of methylated and ethylated pararosanilins and rosanilins, having fewer than five ethyl or methyl groups. Of these compounds, the formula for tri-ethyl rosanilin is: CI I 1 ^ H. H C26H32N3CI; Mol. Wt. {A basic dye ). Please note that
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